苦蔘 추출물이 접촉피부염이 유발된 생쥐의 피부에 미치는 영향 = Effects of Sophorae Radix on skin condition in mice with contact dermatitis induced by Dinitrofluorobenzene

다국어 초록 (Multilingual Abstract)

Objectives: The dried root of Sophora flavescens Aiton have been used to treat patient with skin diseases such as eczema in Eastern countries. S. flavescens can clear away heat and dry dampness and purge sthenic-fire from the liver and gallbladder. Recently, anti-allergic effect of S. flavescens has been reported. However, the effect on skin condition of contact dermatitis still remains unknown. Thus, the purpose of this study is to evaluate the efficacy of S. flavescens on contact dermatitis. Methods : In order to investigate the effects of S. flavescens (methanol extract of S. flavescens, MESR) on skin condition, the contact dermatitis was induced in mice skin by using 1-fluoro-2,4-dinitrofluorobenzene (DNFB). As the effective endpoints, skin thickness, skin weight, histopathological change were checked. In addition, effects on skin lesion, body weight and spleen/body weight ratio were also investigated. Results : Topical application of MESR (500 ㎍/day) lowered skin thickness (P < 0.05) and skin weight (P < 0.05), respectively. MESR-treated group showed diminished spongiosis and immune cell infiltration in skin tissues compared to those of non-treated control group. The inhibition of skin lesions was also observed in MESR-treated group. In addition, MESR did not affect body weight gain and spleen / body weight ratio in contrast with those in dexamethasone-treated group. Conclusion : These data suggest that Sophorae Radix could improve skin lesion of contact dermatitis. This indicates the possibility for Sophorae Radix to be used to patients with skin diseases such as contact dermatitis.

참고문헌 (Reference)

1 안연순, "최근 5년간(1999년-2003년) 산업재해로 요양승인된 직업성 피부질환의 특성" 대한피부과학회 43 (43): 331-341, 2005

2 배유인, "접촉피부염 환자에서 최근 5년간 표준항원 첩포시험 성적" 대한피부과학회 47 (47): 1227-1235, 2009

3 박지혜, "미용사의 직업성 피부질환: 첩포검사 결과에 대한 고찰" 대한피부과학회 44 (44): 669-674, 2006

4 Hong MH, "Sophora flavescens Aiton inhibits the production of pro-inflammatory cytokines through inhibition of the NF kappaB/IkappaB signal pathway in human mast cell line (HMC-1)" 23 (23): 251-258, 2009

5 Liu T, "Matrine inhibits proliferation and induces apoptosis of pancreatic cancer cells in vitro and in vivo" 33 (33): 1740-1745, 2010

6 Piao XL, "Identification and characterization of antioxidants from Sophora flavescens" 29 (29): 1911-1915, 2006

7 Kanda N, "Histamine enhances the production of granulocyte -macrophage colony-stimulating factor via proteinkinase Calpha and extracellular signal-regulated kinase in human keratinocytes" 122 : 863-872, 2004

8 Wen MC, "Efficacy and tolerability of anti-asthma herbal medicine intervention in adult patients with moderate-severe allergic asthma" 116 (116): 517-524, 2005

9 Kim H, "Effect of Sophora flavescens Aiton extract on degranulation of mast cells and contact dermatitis induced by dinitrofluorobenzene in mice" 142 (142): 253-258, 2012

10 KDA Texbook Editing Board, "Dermatology" Ryo Moon Gak 166-167, 2008

11 Smith JG Jr, "Corticosteroid-induced cutaneous atrophy and telangiectasia. Experimental production associated with weight loss in rats" 112 (112): 1115-1117, 1976

12 The textbook compilation committee of Korean herbology, "Bonchohak" Younglimsa 735-, 2010

13 Jin JH, "Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens" 127 (127): 589-595, 2010