Molecular Cloning of a cDNA Encoding a Ribulose - 1 , 5 - Bisphosphate Carboxylase Small Subunit in soybean = Molecular Cloning of a cDNA Encoding a Ribulose - 1 , 5 - Bisphosphate Carboxylase Small Subunit in soybean

다국어 초록 (Multilingual Abstract)

Light enhances the expression of soybean rbcS genes, which encode small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenases. To isolate soybean rbcS genes, a soybean λ cDNA library was constructed with mRNA from light-grown soybean seedlings. The cDNA library was screened using a pea rbcS clone as a probe, and several rbcS ≤DNA clones were isolated. The largest insert DNA from the rbcS λ cDNA clones was subcloned into pBluescribe+, and designated pGSS8. Nucleotide sequence analysis showed that the pGSS8 has a full-length cDNA insert containing 31 nucleotides of 5` untranslated region. The pGSS8 contains an open reading frame of 178 amino acids, including a transit peptide of 55 amino acids. When RNA samples from various soybean tissues were prepared and subjected to Northern blot hybridization, it was shown that the soybean rbcS genes are expressed in green tissues, particularly in leaves.