뇌 해마의 복측 또는 배측 부분이 제거된 흰쥐 중격핵의 신경전달물질 = Neurotransmitter in the Septal Nucleus of Rat with Ventral and Dorsal Hippocampal Ablation

다국어 초록 (Multilingual Abstract)

This study was planned to analyze amino acids in the septal nucleus of dorsal and ventral hippocampectomized rats by high performance liquid chromatography (HPLC). Male albino rats were used. Under chloral hydrate anesthesia (20%, 2 ml/kg body weight), a hole was made in each parietal bone about 4 mm lateral to the sagittal suture, and 4 mm rostral to the lambda suture. The cortical tissue on each side was removed and, through this opening, the hippocampal tissue anterodorsal and posteroventral to the hippocampal flexures on both sides was removed by aspiration. This preparation served as the dorsal and the ventral hippocampal animals, respectively. The cortical control animal received the same surgery short of hippocampectomy. The normal rats served as normal control animal. One day later, the animals were sacrificed by decapitation in the cold room. Two to three milligrams of tissue was obtained form the septal nucleus in one side of the brain. The tissue samples were homogenized and centrifuged. Then content of each amino acid was measured by HPLC form the brain tissue.
The contents of aspartate and glutamate were decreased significantly more in the dorsal and ventral hippocampal groups than in the normal and the cortical control groups. The contents of glutamate were decreased significantly more in the ventral hippocampal group than in the dorsal hippocampal, while there were no significant differences between the two control groups.
It is inferred form the above mentioned results that glutamate and aspartate may be used as excitatory transmitters in septal nucleus, and that the dorsal hippocampus may be facilitatory to the septal nucleus, but the ventral hippocampus would be inhibitory/facilitatory to the cortex.