산느타리(Pleurotus pulmonarius) 품종의 초위성체(simple sequence repeats) 특성구명 = Characterization of simple sequence repeats (SSRs) in Pleurotus pulmonarius cultivars

국문 초록 (Abstract)

국내의 주요 산느타리 품종인 호산47(일핵, 산타리 배우자), GB19(일핵, 산타리 배우자), 호산, 여름느타리1호, 삼복, 강산, 약산, 자산, 향산, 여름느타리2호의 유전체를 Hiseq을 이용하여 해독하였고 이 서열 정보에서 SSR을 분리하여 특성구명을 하였다. 일핵균사인 호산 47, GB19의 유전체의 크기는 각각 37.3와 37.2 Mbp이고, 이핵균사인 나머지 산느타리 품종의 유전체 크기는 47.1~61.1 Mbp인 것으로 밝혀졌다. 품종별 총 SSR의 수는 HS47이 711개로 가장 적고, 강산이(GS)이 1.5배 많은 1,106개로 최다를 기록하였다. SSR의 repeat motif 중에서 hexanucleotide와 octanucleotide가 가장 많은 빈도로 관찰되었고, 가장 많이 관찰되는 반복서열은 CGA/TCG, A/T, CTC/GAG이었다. SSR의 길이는 모든 품종에서 변이가 많아 유용성이 높은 20~30 nt가 가장 높은 비중인 70%를 차지하였다.

다국어 초록 (Multilingual Abstract)

Simple sequence repeats (SSRs) were isolated from major Pleurotus pulmonarius cultivars in Korea, namely 'HS47' (monokaryon, gamete of 'Santari'), 'GB19' (monokaryon, gamete of 'Santari'), 'Hosan,' 'Yeoleumneutali1,' 'Sambok,' 'Gangsan,' 'Yaksan,' 'Jasan,' 'Hyangsan,' and 'Yeoleumneutali2,' and characterized via HiSeq genome sequencing and bioinformatic analysis. The genome sizes of the monokaryons 'HS47' and 'GB19' were estimated to be 37.3 and 37.2 Mb, respectively, and those of the other dikaryotic cultivars ranged from 47.1 to 61.1 Mb. A total of 711 (smallest) and 1,106 (1.5 times the smallest) SSRs were found in the 'HS47' and 'Gangsan' genomes, respectively. Hexanucleotide and octanucleotide motifs accounted for the top two fractions of all SSRs. CGA/TCG, A/T, and CTC/GAG were the most frequently detected nucleotides in the SSRs. Most of the SSRs were 21~30 nucleotides long (hypervariable for application), accounting for 70% of all lengths of SSRs.

참고문헌 (Reference)

1 최종인, "다수성 병재배용 산느타리 신품종 ‘산타리’의 균사배양 및 생육특성" 한국버섯학회 15 (15): 190-194, 2017

2 박보경, "느타리버섯 품종 ‘흑타리’와 ‘미소’의 초위성체 특성구명" 한국버섯학회 14 (14): 174-178, 2016

3 Park B, "Variablenumber tandem repeat loci-discriminating Pleurotus ostreatus cultivars" 60 : 132-135, 2019

4 Zimin AV, "The first near-complete assembly of the hexaploid bread wheat genome, Triticum aestivum" 6 : gix097-, 2017

5 Shnyreva AA, "The commercially cultivated edible oyster mushrooms Pleurotus sajor-caju and P. pulmonarius are two separate species, similar in morphology but reproductively isolated" 48 : 1080-1088, 2012

6 Sharma VP, "Taxonomy, phylogeny, cultivation and biological activities of a Lentinus species from Andaman & Nicobar islands(India)" 27 : 570-576, 2015

7 Karaoglu H, "Survey of simple sequence repeats in completed fungal genomes" 22 : 639-649, 2005

8 Labbe J, "Survey and analysis of simple sequence repeats in the Laccaria bicolor genome, with development of microsatellite markers" 57 : 75-88, 2011

9 Khush GS, "Rice Genetics Iv-Proceedings Of The Fourth International Rice Genetics Symposium (Vol. 4)" World Scientific 122-, 2008

10 Castillo-Lizardo M, "Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi" 5 : 403-, 2014

11 Han SK, "Recorded mushrooms in Ulleungdo and Dokdo, Korea"

12 Lee NK, "New cultivar of Pleurotus pulmonarius(Fr. Quelet), Jasan" 20 : 188-188, 2016

13 Li Y-C, "Microsatellites within Genes : Structure, Function, and Evolution" 21 : 991-1007, 2004

14 Zmitrovich IV, "Is Widely Cultivated" Pleurotus sajor-caju", Especially in Asia, Indeed an Independent Species?" 18 : 583-588, 2016

15 Peona V, "How complete are “complete” genome assemblies?—An avian perspective" 18 : 1188-1195, 2018

16 Guzman G, "Genus Pleurotus (Jacq.: Fr.) P. Kumm. (Agaricomycetideae): diversity, taxonomic problems, and cultural and traditional medicinal uses" 2 : 95-l23, 2000

17 Boekhout T, "Flora agaricina neerlandica" CRC press 20-40, 1990

18 Riley R, "Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi" 111 : 9923-9928, 2014

19 Thiel T, "Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.)" 106 : 411-422, 2003

20 El-Rodeny W, "Development of EST-SSR markers and construction of a linkage map in faba bean(Vicia faba)" 64 : 252-263, 2014

21 Lee KJ, "Cultural characteristics and consumer acceptance of Pleurotus pulmonarius" 6 : 146-149, 2008

22 Im CH, "Construction of a genetic linkage map and analysis of quantitative trait loci associated with the agronomically important traits of Pleurotus eryngii" 92 : 50-64, 2016

23 Ryu J-S, "Complete Genome Sequence of Pleurotus eryngii KNR2312 Using the Next Generation Sequencing (NGS)" 2012

24 Frey KG, "Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood" 15 : 1-14, 2014

25 Park YH, "Breeding of new Grifolaria frondosa, “Dae Whang” and Pleurotus pulmunarius, Hosan and their cultivational properties" 6 : 86-86, 2008

26 Kim KS, "Breeding of Pleurotus sajor-caju cultivar with high yield and adaptable to high temperature" 9 : 22-22, 1997

27 Hwa-Yong Lee, "Analysis of Genetic Diversity and Population Structure of Wild Strains and Cultivars Using Genomic SSR Markers in Lentinula edodes" 한국균학회 48 (48): 115-121, 2020

28 Song Q, "Abundance of SSR motifs and development of candidate polymorphic SSR markers(BARCSOYSSR_1. 0)in soybean" 50 : 1950-1960, 2010

29 Bindler G, "A high density genetic map of tobacco(Nicotiana tabacum L. )obtained from large scale microsatellite marker development" 123 : 219-230, 2011

30 Miah G, "A Review of Microsatellite Markers and Their Applications in Rice Breeding Programs to Improve Blast Disease Resistance" 14 : 22499-22528, 2013