Tryophagus putrescentiae induces inflammatory cytokine secretions from the human cell lines THP-1 and EoL-1 cells

다국어 초록 (Multilingual Abstract)

The present study aimed to identify the roles of the storage mite Tyrophagus putrescentiae (Tp) in relation to human skin diseases, such as atopic dermatitis. We examined the effects of T. putrescentiae extracts (TpE) on the secretion of inflammatory cytokines and their mRNA expression in human immune cells. Human monocytic THP-1 and eosinophilic EoL-1 cells were challenged with TpE and the inflammatory cytokines, interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1) secreted in culture supernatant were analyzed using an enzyme-linked immunosorbent assay (ELISA). The mRNA levels in the cell cultures were also measured via reverse transcriptase-polymerase chain reaction (RT-PCR). In the THP-1 cells, the TpE significantly induced the secretion of IL-6, IL-8, and MCP-1 in a time- and dose-dependent manner. It also stimulated increases in the expression levels of the IL-6, IL-8, and MCP-1 mRNA. However, while the TpE induced IL-8 and MCP-1 mRNA levels in the EoL-1 cells, it did not induce IL-6 expression. Using Western blot analysis, we confirmed the activation of extracellular signal-regulated kinase (ERK), a member of the mitogen-activated protein kinases (MAPKs), in response to TpE challenge of the THP-1 cells.
Collectively, our results show that TpE challenge elicits inflammatory response in the human monocytic THP-1 and eosinophilic EoL-1 cells, and it induces increased production of the inflammatory cytokines IL-6, IL-8, and MCP-1.

참고문헌 (Reference)

1 Cui Y, "Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae" 71 : 1635-1639, 2016

2 Auwerx J, "The human leukemia cell line, THP-1 : a multifaceted model for the study of monocyte-macrophage differentiation" 47 : 22-31, 1991

3 Rothenberg ME, "The eosinophil" 24 : 147-174, 2006

4 Foster PS, "Targeting eosinophils in asthma" 8 : 585-590, 2008

5 이지숙, "Secretion of MCP-1, IL-8 and IL-6 Induced by House Dust Mite, Dermatophagoides pteronissinus in Human Eosinophilic EoL-1 Cells" 한국통합생물학회 13 (13): 391-397, 2009

6 Jõgi NO, "Prevalence of allergic sensitization to storage mites in Northern, Europe" 00 : 1-11, 2019

7 Jeong KY, "Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae" 14 : 431-436, 2007

8 Fernandez-Caldas E, "Molecular and biochemical properties of storage mites(except Blomia species)" 14 : 954-959, 2007

9 Duek L, "Mites in fungal cultures" 44 : 390-394, 2001

10 Mullen GR, "Medical and Veterinary Entomology" Academic Press 423-482, 2009

11 Terakawa M, "Mast cell chymase induces expression of chemokines for neutrophils in eosinophilic EoL-1 cells and mouse peritonitis eosinophils" 538 : 175-181, 2006

12 Mukaida N, "Interleukin-8(IL-8)and monocyte chemotactic and activating factor(MCAF/MCP"1), chemokines essentially involved in inflammatory and immune reactions" 9 : 9-23, 1998

13 Hirano T, "Interleukin-6 and its receptor : ten years later" 16 : 249-284, 1998

14 Gabay C, "Interleukin-6 and chronic inflammation" 8 : 1-3, 2006

15 Steube KG, "Induction and secretion of the chemokines interleukin-8 and monocyte chemotactic protein-1 in human immature leukemia cell lines" 3 : 60-65, 2000

16 Jeong KY, "Immunoglobulin E reactivity of recombinant allergen Tyr p 13 from Tyrophagus putrescentiae homologous to fatty acid binding protein" 12 : 581-585, 2005

17 Munhbayarlah S, "Identification of Tyrophagus putrescentiae allergens and evaluation of cross-reactivity with Dermatophagoides pteronyssinus" 39 : 109-115, 1998

18 Fassio F, "House dust mite-related respiratory allergies and probiotics : a narrative review" 16 : 15-, 2018

19 Lee JS, "House dust mite, Dermatophagoides pteronissinus increases expression of MCP"1, IL-6, and IL-8 in human monocytic THP-1 cells" 42 : 365-371, 2008

20 Yu S-J, "House dust mite allergy : environment evaluation and disease prevention" 4 : 241-252, 2014

21 Arlian LG, "House dust and storage mite extracts influence skin keratinocyte and fibroblast function" 145 : 33-42, 2008

22 정경용, "House Dust Mite Allergy in Korea: The Most Important Inhalant Allergen in Current and Future" 대한천식알레르기학회 4 (4): 313-325, 2012

23 Vidal C, "High prevalence of storage mite sensitization in a general adult population" 59 : 401-405, 2004

24 Harada A, "Essential involvement of interleukin-8(IL-8)in acute inflammation" 56 : 559-564, 1994

25 Hogan SP, "Eosinophils : biological properties and role in health and disease" 38 : 709-750, 2008

26 Mayumi M, "EoL-1, a human eosinophilic cell line" 7 : 243-250, 1992

27 Wong CK, "Differentiation of a human eosinophilic leukemic cell line, EoL-1 : characterization by the expression of cytokine receptors, adhesion molecules, CD95 and eosinophilic cationic protein(ECP)" 68 : 317-323, 1999

28 Homey B, "Cytokines and chemokines orchestrate atopic skin inflammation" 118 : 178-189, 2006

29 Bellanti JA, "Cytokines and allergic diseases: clinical aspects" 19 : 337-341, 1998

30 Solarz K, "Comparisons of the allergenic mite prevalence in dwellings and certain outdoor environments of the Upper Silesia(southwest Poland)" 210 : 715-724, 2007

31 Auwerx J, "Changes in IgG Fc receptor expression induced by phorbol 12-myristate 13-acetate treatment of THP-1 monocyte leukemia cells" 16 : 317-327, 1992

32 Arlian LG, "Antigenic and allergenic properties of the storage mite Tyrophagus putrescentiae" 74 : 166-171, 1984

33 Arlian LG, "Allergenic characterization of Tyrophagus putrescentiae using sera from occupationally exposed farmers" 79 : 525-529, 1997

34 Nuñez NK, "Acute and chronic exposure to Tyrophagus putrescentiae induces allergic pulmonary response in a murine model" 6 : 48-55, 2016