밀겨 유래의 3T3-L1 세포 지방생성 억제물질 = Inhibitors of Adipogenesis in 3T3-L1 Cells Isolated from Wheat Bran

다국어 초록 (Multilingual Abstract)

As a part of our ongoing program on finding biologically active components from natural source we found three known constituents from the EtOH extract of the wheat bran. The known compounds were identified as tachioside (1), pinellic acid (2) and tryptophan (3). The structure and relative stereochemistry were determined from MS, 1D and extensive 2D NMR techniques as well as by comparison of their data with the published values. All isolates were tested their inhibitory effects on the adipogenesis in 3T3-L1 cells. The effect of compounds from wheat bran on 3T3-L1 adipocyte differentiation were measured by Oil Red O staining. These results demonstrate that tachioside (1) and pinellic acid (2) decreased lipid content in 3T3-L1 adipocytes by inhibiting lipogenesis. These compounds had shown antiobesity activities.

참고문헌 (Reference)

1 이기택, "선택된 식물자원에서 추출조건에 따른 Phytosterol과 Tocopherol의 함량분석 및 추출유로부터 재구성지질의 합성" 한국식품저장유통학회 10 (10): 370-375, 2003

2 김미리혜, "비만치료의 최신 지견 : 심리적 접근을 중심으로" 한국건강심리학회 9 (9): 493-510, 2004

3 Morrison, W. R., "Wheat lipid composition" 55 : 548-558, 1978

4 Gregoire FM, "Understanding adipocyte differentiation" 78 : 783-809, 1988

5 Gutsche, B., "Tryptophan glycoconjugates in food and human urine" 343 : 11-19, 1999

6 Rosen, E. D., "Transcriptional regulation of adipogenesis" 14 : 1293-1307, 2000

7 Bradford, B. L., "Towards a molecular understanding of adaptive thermogenesis" 404 : 652-660, 2000

8 Griffiths, D. W., "The inhibition of digestive enzymes by polyphenolic compounds" 1999 : 509-516, 1986

9 Ting Li, "Tachioside, an Antioxidative Phenolic Glycoside from Bamboo Species" 한국식품과학회 17 (17): 1376-1378, 2008

10 Maes, C., "Structural characterization of water-extractable and water-unextractable arabinoxylans in wheat bran" 35 : 315-326, 2002

11 Iwatsuki, K., "Sterol ferulates, sterols, and 5-alk(en)ylresorcinols from wheat, rye, and corn bran oils and their inhibitory effects on Epstein-barr virus activation" 51 : 6683-6688, 2003

12 Dimitrios, B., "Sources of natural phenolic antioxidants" 17 : 505-512, 2006

13 Wang, J., "Protein glycation inhibitory activity of wheat bran feruloyl oligosaccharides" 112 : 350-353, 2009

14 Carl, H. R., "Principles of cereal science and technology" American Association of Cereal Chemists 1-10, 1990

15 Reddy, B. S., "Preventive potential of wheat bran fractions against experimental colon carcinogenesis: implications for human colon cancer prevention" 60 : 4792-4797, 2000

16 Nagai, T., "Pinellic acid from the tuber of Pinellia ternata Breitenbach as an effective oral adjuvant for nasal influenza vaccine" 2 : 1183-1193, 2002

17 Oueslati, M. H., "Phytochemical constituents from Salsola tetrandra" 69 : 1366-1369, 2006

18 Seo, Y. H., "Patent trend of anti-obesity supplementary food" 8 : 116-122, 2005

19 Albu, J., "Obesity solutions report of a meeting" 55 : 150-156, 1997

20 Kim, J. B., "New horizon in atherosclerosis research: Insights into fat cell differentiation and insulin sensitivity with ADD1/SREBP1 and PPARγ" 9 : 79-83, 2001

21 Grundy, S. M., "Multifactorial causation of obesity: implications for prevention" 67 : 563S-572S, 1998

22 Lew, E. A., "Mortality and weight: insured lives and the American Cancer Society studies" 103 : 1024-1029, 1985

23 Michio, S., "Lipoapoptosis in beta-cells of obese prediabetic fa/fa rats" 273 : 32487-23490, 1998

24 Qu, H., "Lignans are involved in the antitumor activity of wheat bran in colon cancer SW480 cells" 135 : 598-602, 2005

25 Antipatis, V. J., "International textbook of obesity" John Wiley & Sons Ltd 3-22, 2001

26 Delzenne, N. M., "Impact of insulin and oligofructose on gastrointestinal peptides" 1 : 157-161, 2005

27 Cukelj, "Identification and quantification of lignans in wheat bran by gas chromatography-electron capture detection" 84 : 127-132, 2011

28 Reddy, P., "Focus on orlistat: A nonsystemic inhibitor of gastrointestinal lipase for weight reduction in the management of obesity" 33 : 943-959, 1998

29 Tontonoz, P., "Fat and beyond: the diverse biology of PPARgamma" 77 : 289-312, 2008

30 Zacour, A. C., "Effect of dietary chitin on cholesterol absorption and metabolism in rats" 38 : 609-613, 1992

31 Burns, A. A., "Does-response effects of a novel fat emulsion (Oliibra) on energy and macronutrient intakes up to 36h post-consumption" 56 : 368-377, 2002

32 Kopelman, P. G., "Causes and consequences of obesity" 22 : 385-388, 1994

33 Baublis, A. J., "Antioxidants in wheat-based breakfast cereals" 45 : 71-74, 2000

34 Wangensteen, H., "Antioxidant and 15-lipoxygenase inhibitory activity of rotenoids, isoflavones and phenolic glycosides from Sarcolobus globosus" 77 : 290-295, 2006

35 Yuan, X., "Antioxidant activity of feruloylated oligosaccharides from wheat bran" 90 : 759-764, 2005

36 Brindzova, L., "Antimutagenic and radical scavenging activity of wheat bran" 37 : 45-55, 2009

37 Nagai, T., "Anti-allergic activity of a Kampo (Japanese herbal) medicine “Sho-seiryu-to (Xiao-Qing-Long-Tang)” on airway inflammation in a mouse model" 4 : 1353-1365, 2004

38 Kim, J. B., "ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism" 10 : 1096-1107, 1996