<P>Although mutations in more than 90 genes are known to cause CMT, the underlying genetic cause of CMT remains unknown in more than 50% of affected individuals. The discovery of additional genes that harbor CMT2-causing mutations increasingly d...
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https://www.riss.kr/link?id=A107467348
Lassuthova, Petra ; Rebelo, Adriana P. ; Ravenscroft, Gianina ; Lamont, Phillipa J. ; Davis, Mark R. ; Manganelli, Fiore ; Feely, Shawna M. ; Bacon, Chelsea ; Brož ; ková ; , Dana Š ; afka ; Haberlova, Jana ; Mazanec, Radim ; Tao, Feifei ; Saghira, Cima ; Abreu, Lisa ; Courel, Steve ; Powell, Eric ; Buglo, Elena ; Bis, Dana M. ; Baxter, Megan F. ; Ong, Royston W. ; Marns, Lorna ; Lee, Yi-Chung ; Bai, Yunhong ; Isom, Daniel G. ; Barro-Soria, René ; Chung, Ki W. ; Scherer, Steven S. ; Larsson, H. Peter ; Laing, Nigel G. ; Choi, Byung-Ok ; Seeman, Pavel ; Shy, Michael E. ; Santoro, Lucio ; Zuchner, Stephan
2018
-
SCI,SCIE,SCOPUS
학술저널
505-514(10쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Although mutations in more than 90 genes are known to cause CMT, the underlying genetic cause of CMT remains unknown in more than 50% of affected individuals. The discovery of additional genes that harbor CMT2-causing mutations increasingly d...
<P>Although mutations in more than 90 genes are known to cause CMT, the underlying genetic cause of CMT remains unknown in more than 50% of affected individuals. The discovery of additional genes that harbor CMT2-causing mutations increasingly depends on sharing sequence data on a global level. In this way—by combining data from seven countries on four continents—we were able to define mutations in <I>ATP1A1</I>, which encodes the alpha1 subunit of the Na<SUP>+</SUP>,K<SUP>+</SUP>-ATPase, as a cause of autosomal-dominant CMT2. Seven missense changes were identified that segregated within individual pedigrees: c.143T>G (p.Leu48Arg), c.1775T>C (p.Ile592Thr), c.1789G>A (p.Ala597Thr), c.1801_1802delinsTT (p.Asp601Phe), c.1798C>G (p.Pro600Ala), c.1798C>A (p.Pro600Thr), and c.2432A>C (p.Asp811Ala). Immunostaining peripheral nerve axons localized ATP1A1 to the axolemma of myelinated sensory and motor axons and to Schmidt-Lanterman incisures of myelin sheaths. Two-electrode voltage clamp measurements on <I>Xenopus</I> oocytes demonstrated significant reduction in Na<SUP>+</SUP> current activity in some, but not all, ouabain-insensitive ATP1A1 mutants, suggesting a loss-of-function defect of the Na<SUP>+</SUP>,K<SUP>+</SUP> pump. Five mutants fall into a remarkably narrow motif within the helical linker region that couples the nucleotide-binding and phosphorylation domains. These findings identify a CMT pathway and a potential target for therapy development in degenerative diseases of peripheral nerve axons.</P>