겨우살이 lectin의 간기능 영향 = Effect of viscum album lectin on rat liver function

다국어 초록 (Multilingual Abstract)

In the present study, the author investigated change of liver funtion in Viscum album lectin The lectin was purified by 0.15 M NaCl extraction, amnonium sulfate precipitation, sepharose 4B affinity chromatography and gel filtration using sephadex G-150 with plant material from Viscum album collected in Mt. Duk Yui.
After 72 h of CCI, injection (in olive oil, 1:1, 2 mg/kg) there was a sig nificant increase in serum AST and ALT levels relative to the control group. However, treatment of either Viscum album extracts and purified lectin offered no protection against CC1,-induced hepatotoxicity. Histological observation basically supported the result obtained from serum enzyme assay. The livers of rats challenged with CCl. produced a marked increased cytoplasmic vacuoles and inflammatory cellsin num-ber, while the number of necrotic cells and swollen hepatocytes did not change significnatly. Rats administered olive oil alone did not alter the normal hepatic architecture. Histological observation of liver section in rat treated 72 h with either Viscum album extract and purified lectin CCl-induced liver damage showed number of cytoplasmic vaculoe and necrotic cell. The number of inflammatory cell increased markedly. The normal hepatic architectural pattern was lost in Hematoxylin-eosin stain.
This results suggest to the conclusion that Viscum album lectin has a physiological effect of hepatotoxicity activator.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 실험재료 및 방법
• Ⅲ. 실험결과
• Ⅳ. 고찰
• Ⅴ. 결과