6-Hydroxydopamine로 유도된 In Vitro 파킨슨병 모델에서 토란추출물의 Brain Resilience에 미치는 영향 = Effects of Taro Extract on Brain Resilience in In Vitro Parkinson’s Disease Model Induced by 6-Hydroxydopamine

다국어 초록 (Multilingual Abstract)

Purpose: The purpose of this study was to investigate the effects of taro extract on brain resilience in in vitro Parkinson’s disease model induced by 6-hydroxydopamine (6-OHDA). Methods: To induce a neuroinflammatory reaction and the in vitro Parkinson’s disease model, SH-SY5Y cells were stimulated with lipopolysaccharide (LPS) and 6-OHDA, respectively. After that, cells were treated with at various concentrations (1, 5, and 10 mg/mL) of taro extract. Then nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), interleukin (IL)-6, synaptophysin (SYP) and growth associated protein (GAP)-43 messenger ribonucleic acid (mRNA) expression level were measured. Results: Taro extract significantly suppressed LPS-induced NO production. Meanwhile, iNOS and IL-6 mRNA expression decreased in a dose-dependent manner. In addition, taro increased the mRNA expression of SYP and GAP-43 mRNA. Conclusion: These findings indicate that taro played an important role in brain resilience by inhibiting neuronal cell death and promoting neurite outgrowth, synaptogenesis, and neural plasticity. The results of this study suggest that taro may contribute to the prevention of neurodegenerative disease and become a new and safe therapeutic strategy for Parkinson’s disease.

목차 (Table of Contents)

• 서 론 연구 방법 연구 결과 논 의 결 론

참고문헌 (Reference)

1 전유호, "흑토란의 식품재료화를 위한 숙성 조건에 따른 토란의 특성 및 관능 최적화" 한국식품과학회 48 (48): 133-141, 2016

2 이지은, "국민건강보험공단 표본 코호트 자료를 이용한 한국인 파킨슨 환자의 유병률 및 발생률" 대한신경과학회 35 (35): 191-198, 2017

3 문지혜, "고지방 식이를 섭취한 마우스에서 토란분말의 비만억제 및 지질저하 효과" 한국식품과학회 42 (42): 620-626, 2010

4 Pragati Kaushal, "Utilization of taro (Colocasia esculenta): a review" Springer Science and Business Media LLC 52 (52): 27-40, 2015

5 Mal-Soon Shin, "Treadmill exercise facilitates synaptic plasticity on dopaminergic neurons and fibers in the mouse model with Parkinson’s disease" Elsevier BV 621 : 28-33, 2016

6 Qifei Cong, "The endogenous neuronal complement inhibitor SRPX2 protects against complement-mediated synapse elimination during development" Springer Science and Business Media LLC 23 (23): 1067-1078, 2020

7 Xi Lu, "The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons" Springer Science and Business Media LLC 9 (9): 17-, 2014

8 Patricia Ribeiro Pereira, "Tarin, a potential immuno-modulator and COX Inhibitor lectin found in taro (Colocasia esculenta)" Wiley 17 (17): 878-891, 2018

9 Sonja Rakic, "Systemic infection modifies the neuroinflammatory response in late stage Alzheimer’s disease" Springer Science and Business Media LLC 6 (6): 88-, 2018

10 Huixian Li, "Structure characterization of two novel polysaccharides from Colocasia esculenta (taro) and a comparative study of their immunomodulatory activities" Elsevier BV 42 : 47-57, 2018

11 Xie HR, "SH-SY5Y human neuroblastoma cell line : in vitro cell model of dopaminergic neurons in parkinson’s disease" 123 (123): 1086-1092, 2010

12 M.K. Barthwal, "Role of nitric oxide in a progressive neurodegeneration model of Parkinson's disease in the rat" Informa UK Limited 6 (6): 297-302, 2013

13 Lorraine V Kalia, "Parkinson's disease" Elsevier BV 386 (386): 896-912, 2015

14 Nico J. Weerkamp, "Nonmotor Symptoms in Nursing Home Residents with Parkinson's Disease: Prevalence and Effect on Quality of Life" Wiley 61 (61): 1714-1721, 2013

15 Zhengliang Gao, "Neurod1 is essential for the survival and maturation of adult-born neurons" Springer Science and Business Media LLC 12 (12): 1090-1092, 2009

16 박건혁, "MPTP로 유도된 Parkinson’s disease 동물 모델에서 항염증효과를 통한 측백엽의 도파민신경보호 효과" 대한본초학회 29 (29): 27-33, 2014

17 Ziyuan Guo, "In Vivo Direct Reprogramming of Reactive Glial Cells into Functional Neurons after Brain Injury and in an Alzheimer’s Disease Model" Elsevier BV 14 (14): 188-202, 2014

18 M. Volosin, "Interaction of Survival and Death Signaling in Basal Forebrain Neurons: Roles of Neurotrophins and Proneurotrophins" Society for Neuroscience 26 (26): 7756-7766, 2006

19 Guy C. Brown, "Inflammatory Neurodegeneration and Mechanisms of Microglial Killing of Neurons" Springer Science and Business Media LLC 41 (41): 242-247, 2010

20 Pia Rivetti di Val Cervo, "Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model" Springer Science and Business Media LLC 35 (35): 444-452, 2017

21 Terri R. Fried, "Health Outcomes Associated with Polypharmacy in Community-Dwelling Older Adults: A Systematic Review" Wiley 62 (62): 2261-2272, 2014

22 Matthew Holahan, "GAP-43 in synaptic plasticity: molecular perspectives" Informa UK Limited 5 : 137-146, 2015

23 Michael T. Heneka, "Expression and function of inducible nitric oxide synthase in neurons" Elsevier BV 114 (114): 8-18, 2001

24 Jess Nithianantharajah, "Enriched environments, experience-dependent plasticity and disorders of the nervous system" Springer Science and Business Media LLC 7 (7): 697-709, 2006

25 Manisha Kalariya, "Effect of hydroalcoholic extract of leaves of colocasia esculenta on marble-burying behavior in mice: Implications for obsessive–compulsive disorder" Informa UK Limited 53 (53): 1239-1242, 2015

26 Hee Kim, "Effect of Red Ginseng on Genotoxicity and Health-Related Quality of Life after Adjuvant Chemotherapy in Patients with Epithelial Ovarian Cancer: A Randomized, Double Blind, Placebo-Controlled Trial" MDPI AG 9 (9): 772-, 2017

27 Stéphane Walrand, "Dietary supplement intake among the elderly: hazards and benefits" Ovid Technologies (Wolters Kluwer Health) 21 (21): 465-470, 2018

28 U. Schmitt, "Detection of behavioral alterations and learning deficits in mice lacking synaptophysin" Elsevier BV 162 (162): 234-243, 2009

29 Xiankun Zhu, "Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats" Ivyspring International Publisher 16 (16): 394-402, 2019

30 So Hyun Ahn, "Bogijetong decoction and its active herbal components protect the peripheral nerve from damage caused by taxol or nerve crush" Springer Science and Business Media LLC 16 (16): 402-, 2016

31 Ezinne Awa, "Bioactive constituents and antioxidant activities of raw and processed cocoyam (Colocasia esculenta)" Springer Science and Business Media LLC 14 (14): 133-140, 2015

32 Robert E. Burke, "Axon degeneration in Parkinson's disease" Elsevier BV 246 : 72-83, 2013

33 Phillip M. Rappold, "Astrocytes and therapeutics for Parkinson’s disease" Springer Science and Business Media LLC 7 (7): 413-423, 2010

34 Hae-Young Park, "Association of Parkinsonism or Parkinson Disease with Polypharmacy in the Year Preceding Diagnosis: A Nested Case–Control Study in South Korea" Springer Science and Business Media LLC 40 (40): 1109-1118, 2017

35 이진숙, "6-OHDA로 유도된 파킨슨 증후군의 동물 모델에서 도파민성 신경세포의 손실과 미세아교세포 활성 사이의 관계" 대한체질인류학회 26 (26): 13-23, 2013

36 이찬, "6-Hydroxydopamine으로 유도된 질소적 세포 사멸에 대한 고려홍삼 추출물의 보호효과" 대한약학회 60 (60): 92-99, 2016