Benzoate 분해균주의 분리 및 특성 = Isolation and Characterization of Benzoate-Degrading Bacteria

다국어 초록 (Multilingual Abstract)

Twenty-seven bacterial colonies capable of growing on benzoate as the sole source of carbon were isolated from streamside soils. Three of isolates were found to be extremely effective in metabolizing the benzoate, when they were incubated in the basal salt medium containing 2,000ppm of benzoic acid at 30℃ for 48hrs. The three isolates, Y205, W212 and J211 were identified Pseudomonas aeruginosa, Bacillus fastidiosus, and Aeromonas sp. respectively through morphological and physiological examination.
Optimal temperature and pH for benzoate degradation of the isolates appeared to be 35℃ and pH for Pseudomonas sp. Y205 and Bacillus. sp. W212, and 30℃ and pH 7.0 for Aeromonas sp. J211. The highest degradation of benzoate by the three isolates was obstained in the growth media containing ammonium sulfate as nitrogen source.
Optimal concentration of ammonium sulfate was 6.0g/l for Pseudomonas sp. Y205 and Aeromonas sp. J211, and 4.0g/l for Bacillus. sp. W212. When the three isolates were cultivated in basal salt medium containing 2,000ppm benzoate under optimal condition, they showed a logarithmic phase growth 12hours and a stationary phase growth 18hours after innoculation, and the benzoate degradation of 91.15-92.40% after 72-hours incubation. Benzoate degradation increased in parallel with the cell growth.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 1. 토양 시료 체취
• 2. 사용배지
• 3. Benzoate 분해균주의 선발
• 4. 균주의 동정
• 5. 분리균주의 benzoate 분해력에 미치는 배양조건
• 6. 최적조건에서 균주의 생육측정
• 결과 및 고찰
• 1. Benzoate 자화성 균주의 선발
• 2. 우수 야생균주의 동정
• 3. Benzoate 분해의 최적조건
• 4. 최적조건에서 균주의 생육 및 benzoate 분해
• 요약