Analysis of DNA methylation in tissues and development stages of pearl oyster Pinctada fucata

다국어 초록 (Multilingual Abstract)

In this study, we developed a methylationsensitiveamplification polymorphism technique to investigateDNA methylation profiles in different tissues and inthe early-development stages of the pearl oyster Pinctadafucata (P. fucata). Methylation levels in adductor muscle,digestive gland, axe foot, heart, and gill ranged from 11.71to 14.71 %, and significant differences (P\0.05) betweenmethylation levels in different tissues were observed. TheDNA methylation levels of sperm, egg cells, two-cellembryos, morula embryos, trochophore larvae andD-shaped larvae were 13.51, 11.80, 12.14, 12.60, 14.65 and13.18 %, respectively. Development stages of two-cellembryos, morula embryos, trochophore larvae andD-shaped larvae indicated a higher number of identicalDNA methylation status loci in the egg, compared to that inthe sperm. It is probable that DNA methylation patterns ofthe progeny are mainly influenced by the egg, while thesperm may become increasingly important during theprocess of early embryo development. The observeddifferences in methylation levels in the tissues and thedevelopment stages of P. fucata suggest that DNA methylationmay act as an epigenetic regulator during tissuedifferentiation, individual growth, and development.

참고문헌 (Reference)

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