Tetracycline 처리된 차폐막이 치은 섬유아세포에 미치는 영향 = The role of tetracycline-treated barrier membrane onhuman gingival fibroblast

다국어 초록 (Multilingual Abstract)

The purpose of this study was to evaluate the effect of tetracycline-treated e-PTFE membrane on the human gingival fibroblast attachment. To evaluate the cell attachment to membrane, the number of gingival fibroblast attached to e-PTFE membrane was counted by hemocytometer. Also the cytotoxic effect of tetracycline-treated e-PTFE membrane on human gingival fibroblast was measured by Wataha's MTT assay. After tetracycline-treated barrier membrane was exposed to culture media for 24 hours, cell attaachment to membrane and cytotoxic effect were measured.
The results were as follows;
As for cell proliferation rate, total cell number increase in both control group and tetracycline-treated e-PTFE membrane in course of time, but the cell numbers were decreased to half in tetracycline-treated barrier membrane as in the control group.
In MTT assay, tetracycline-treated e-PTFE membrane showed moderate to weak cytotoxic effect compared to non-treated control. At the begining, tetracycline-treated, so was barrier membrane released abundant tetracycline and so decreased the number of cell attached barrier membrane, but not almost observed cellular activity inhibition on attached cell and as decreasing tetracycline-treated barrier membrane, the number of attached cell were increased.
These results might suggest that tetracycline-treated e-PTFE membrane showed relatively low proliferation rate of attached cells, but weak cytotoxic effect. So it is suggested tetracycline-treated e-PTFE membrane could bind gingival connective tissue normally and the healing site could gain stability.

목차 (Table of Contents)

• Ⅰ. 서 론
• Ⅱ. 재료 및 방법
• Ⅲ. 결 과
• Ⅳ. 고 찰
• Ⅴ. 결 론
• 참고문헌
• 영문초록