Glutamate가 유도하는 세포독성으로부터 신경세포를 보호하는 상백피 추출물의 효과

국문 초록 (Abstract)

본 연구에서는 glutamate로 유도된 세포독성으로부터 신경세포를 보호하는 상백피(Morus alba) 추출물의 활성을 확인하기 위하여 N18-RE-105 세포주를 이용하여 MTT reduction assay, LDH release assay 및 광학 현미경을 이용하여 형태학적인 변화를 관찰하였다. 그 결과, 상백피 methanol 추출물에서 농도 의존적으로 신경세포 보호효과가 나타났으며, 50 ㎍/㎖ 농도에서는 80% 이상의 세포생존율을 확인할 수 있었다. 이 결과는 N18-RE-105 세포주의 LDH release assay와 형태학적 변화에서도 일치하는 결과를 확인하였다. 가장 높은 활성을 보인 상백피 methanol 추출물을 hexane, diethyl ether, ethyl acetate, water 층으로 분획하여 각 각 1, 5, 10 ㎍/㎖ 농도로 처리 시 hexane 층에서 48.0%, 65.6%, 71.5%로 가장 높은 신경세포 보호효과를 확인할 수 있었다. 따라서 상백피 추출물은 glutamate에 의한 세포독성으로부터 신경세포 손상을 억제하며 신경세포를 보호하는 효과가 있다는 것을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

This study evaluated the neuroprotective effect of extracts from the root bark of Morus alba (MA) against glutamate-induced cytotoxicity in neuronal cells. Glutamate-induced cytotoxicity was shown by MTT reduction assay. The neuroprotective effects of methanol, ethanol, and acetone extracts from MA against glutamate-induced cytotoxicity were measured. Among the three extracts, the methanolic extracts showed the highest protective effect, as determined by the results of an morphological assay, a lactate dehydrogenase release assay. Furthermore, the methanol extracts were fractionated sequentially with hexane, diethyl ether, ethyl acetate, and water layer according to degree of polarity. The hexane fractions exhibited a neuroprotective effect against glutamate-stressed N18-RE-105 cells. Therefore, these results suggest that extracts of MA could be a new potential candidate as a protective substance against glutamate-induced cytotoxicity.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• References

참고문헌 (Reference)

1 황용일, "참곱슬이(Plocamium telfairiae) 추출물의 암세포 성장억제 효과" 한국생명과학회 16 (16): 659-663, 2006

2 윤미영, "석곡MeOH 추출물이 H2O2에의한신경세포 보호효과에 미치는 영향" 한국응용생명화학회 50 (50): 63-67, 2007

3 Kim, S. Y, "protective effets of extracts of Mori Cortex Radicis on corbon tetrachloride-induced hepatotoxicity" 43 : 391-396, 1999

4 Jin, D. Q, "nti-oxidant and anti-inflammatory activity of macelignan in murine hippocampal cell line and primary culture of rat microglial cells" 331 : 1264-1269, 2005

5 Duffy, S, "ctivation of endogenous antioxidant defenses in neuronal cells prevents free radical-madiated damage" 71 : 69-77, 1998

6 Hara, J., "Thyrotropin-releasing hormone increases behavioral arousal through modulation of hypocretin/orexin neurons" 29 : 3705-3714, 2009

7 Monaghan, D. T, "The excitatory amino acid receptors: their classes, pharmacology and distinct properties in the function of the central nervous system" 29 : 365-402, 1989

8 Harman,D, "The aging process" 78 : 7124-7128, 1981

9 Chen, L, "Sustained potentiation of NMDA receptor-mediated glutamate responses through activation of protein kinase C by a mu opioid" 7 : 319-326, 1991

10 Cowburn, R, "Regional distribution of pre- and postsynaptic glutamatergic function in Alzheimer’s disease" 452 : 403-407, 1988

11 Choi, B. W, "Protein kinase Cδ-mediated proteasomal degradation of MAP kinase phosphatase-1 contributes of glutamate-induced neuronal cell death" 119 : 1329-1340, 2005

12 Nomura, T, "Phenolic constituents of the cultivated Mulberry tree (Morus alba L.)" 24 : 2898-2900, 1976

13 Coyle, J. T, "Oxidative stress, glutamate, and neurodegenerative disorders" 262 : 689-695, 1993

14 Dillon-Carter, O, "N18-RE-105 cells: differentiation and activation of p53 in response to glutamate and adriamycin is blocked by SV40 large T antigen tsA58" 291 : 191-205, 1998

15 Hikino, H, "Isolation and hypoglycemic activity of Moran A, a glycoprotein of Mousalba root barks" 51 : 159-160, 1985

16 Weiloch,T, "Hypoglycemai-induced neuronal damage prevented by an N-methyl-D-aspartate antagonists" 230 : 681-683, 1985

17 Zhang, W., "HPLC-DAD-ESI-MS/MS analysis and antioxidant activities of nonanthocyanin phenolics in mulberry (Morus alba L.)" 73 : 512-518, 2008

18 이미라, "Glutamate에 의한 산화적 스트레스로부터 신경세포를 보호하는제비꽃 추출물의 영향" 한국응용생명화학회 51 (51): 79-83, 2008

19 Fonnum, F, "Glutamate: a neurotransmitter in mammalian brain" 42 : 1-11, 1984

20 Parfenova, H, "Glutamate induces oxidative stress and apoptosis in cerebral vascular endothelial cells: contributions of HO-1 and HO-2 to cytoprotection" 290 : 1399-1401, 2005

21 Olanwo, C. W, "Etiology and pathogenesis of Parkinson’s disease" 22 : 123-144, 1999

22 Kang, T. H, "Enhancement of neuroprotection of mulberry leaves (Morus alba L.) prepared by the anaerobic treatment against ischemic damage" 29 : 270-274, 2006

23 D'Orlando, C, "Calretinin and calbindin D-28k, but not parvalbumin protect against glutamate-induced delayed excitotoxicity in trasfected N18-RE 105 neuroblastoma-retina hybrid cells" 945 : 181-190, 2002

24 Lim, C. S, "Antioxidant and anti-inflammatory activities of the nethanolic extract of neorhodomela aculeate in hippocampal and microglial cells" 29 : 1212-1216, 2006

25 Kim, M. J, "Activation effects of allium tuberosum Rottl. on choline acetyltrasnferase" 71 : 226-230, 2007

26 Noh, H. S, "Acetoacetate protects neuronal cells from oxidative glutamate toxicity" 83 : 702-709, 2006