Investigation of the Efficiency of Direct and Indirect Regeneration in Evening Primrose (Oenothera biennis)

다국어 초록 (Multilingual Abstract)

As a medicinal plant, the importance of evening primrose (Oenothera biennis L.) is due to its unsaturated fatty acids in the seedsand roots, and also oenotherine and comfarol in the leaves. Low germination and difficulties in seed production are the main problemsencountered with growing this plant in the field. As an alternative approach, an in vitro experiment was set up for the evaluationof evening primrose production via direct and indirect regeneration of the cultivars NC-1 and VNK. For callogenesis and directregeneration, the explants from the apical bud and petiole were cultured on MS medium supplemented with 0.25, 0.75, and 1.25 mgL-1 of both BAP and Kinetin (KIN). Indirect regeneration was performed by placing apical buds, petioles, and leaf explants on MSmedium supplemented with 0.5 and 1 mg L-1 2,4-D and 0.5, 1, and 1.25 mg L-1 of both BAP and KIN. The highest shoot inductionfrom direct regeneration was obtained with apical bud explants of VNK treated with 0.75 mg L-1 BAP. The highest callus weight(3.17 g) obtained from indirect regeneration was with petiole explants treated with 1 mg L-1 2, 4-D and 1 mg L-1 BAP in VNK cultivars.
The highest number of torpedo embryogenic clusters (23.8) was obtained from the VNK petiole explants treated with 0.5 mg L-12, 4-D and 1.25 mg L-1 BAP. BAP had higher positive effects on in vitro production of evening primrose than KIN in both direct andindirect regeneration. In general, results indicated that VNK was more potent for regeneration than NC-1 and concentrations of 0.75mg L-1 BAP for direct and 0.5 mg L-1 2, 4-D and 1.25 mg L-1of BAP for indirect regeneration had a higher efficiency for increasing invitro production of evening primrose

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