Effects of moderate pressure on premeability and viability of Saccharomyces cerevisiae cells

다국어 초록 (Multilingual Abstract)

With CO2 and N2 as the pressure media, the effects of the moderate pressure (0.1-1.0MPa) and the holding
time on the conductivities of the cell suspension of Saccharomyces cerevisiae CICC1447 and Saccharomyces cerevisiae
CICC1339, as well as the absorbances of the supernatant (after centrifuged) at 280 nm (A280) and 260 nm (A260) were
determined. The membrane permeability of Saccharomyces cerevisiae CICC1447 increased significantly and the cell
leakage was aggravated with the pressure increase. For Saccharomyces cerevisiae CICC1339, the conductivity of the
cell suspension, A280 and A260 of the supernatant fluctuated with the pressure increase; as a whole, they increased with
pressure. Different from high pressure, a moderate pressure not only remarkably improved the permeability of the yeast
cell membrane, but also kept yeast cell viability; moreover, the integrity of the yeast cell membrane could be maintained.

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