다공크기에 따라 수핵세포가 파종된 PLGA 지지체의 압축강도에 관한 연구 = Compressive Strength of Poly(L-Lactide-co-glycolide) Scaffolds Seeded Nucleus Pulposus Cells Depending on Pore Size

다국어 초록 (Multilingual Abstract)

Tissue engineering has the possible to improve upon current techniques for intervertebral disc repair. A key component in tissue engineering for disc regeneration is the scaffold that serves as a template for cell interaction and the formation of extracellular matrix to provide structural support to the newly formed tissue. Recently developed scaffolds, Poly(lactide-co-glycolide)(PLGA) most widely used due to its advantages such as good biocompatibility, controllable rate of degradation and metabolizable degradation products. We measured compression strength of scaffolds with various pores to select suitable pore for tissue-engineering bio-disc. PLGA scaffolds were prepared by solvent casting/salt-leaching. NP cell were harvested from the disc of adult female rabbit. These cells were seeded in prepared PLGA scaffold and cultured in DMEM mdeium supplimented, 10% FBS, 1% antibiotic antinycotics for 1, 4, 6 weeks. We Morphology of cellular adhesion and extracellular matrix production were confirmed by scanning electron microscope(SEM). According to our result, the compression strength of scaffold decreased with increasing porogen size.

참고문헌 (Reference)

1 고연경, "히알루론산을 함유한 PLGA 지지체의 제조 및 특성결정" 한국고분자학회 31 (31): 505-511, 2007

2 최방실, "탈미네랄화 골분(DBP)이 PLGA 지지체의 염증반응을 완화시킨다" 한국조직공학과 재생의학회 3 (3): 295-300, 2006

3 강길선, "중추신경재생 연구의 최근 동향" 한국조직공학과 재생의학회 1 (1): 136-142, 2004

4 장지욱, "조직공학적 골재생을 위한 탈미넬화된 골분을 함유한 다공성 지지체의 제조 및 그 특성" 한국고분자학회 28 (28): 382-390, 2004

5 강길선, "인공 디스크 연구 개발의 현황" 한국조직공학과 재생의학회 2 (2): 20-28, 2005

6 고연경, "인간 디스크세포와 PLGA/DBP 지지체를 이용한 생체조직공학적 바이오 디스크" 한국조직공학과 재생의학회 4 (4): 67-72, 2007

7 하현정, "섬유륜과 수핵 세포의 계대에 따른 특성분석" 한국조직공학과 재생의학회 3 (3): 416-422, 2006

8 G Khang, "Scaffolds; Tissue engineering, In Webser's Biomedical Engineering Handbook, in : 2ng Eds" 1 : 336-, 2006

9 G Khang, "Scaffolds for tissue engineering" 3 : 376-, 2004

10 JC Cho, "Preparation of biodegradable PLGA microspheres for sustainde local anesthesia and their in vitro release behavior" 24 : 728-, 2000

11 EK Jeon, "Preparation and release profile of NGF-loaded polylacide scaffolds for tissue engineered nerve regeneration" 25 : 893-, 2001

12 소정원, "PLGA미립구의 크기에 따른 인간디스크세포의 증식 및 표현형 분석" 한국조직공학과 재생의학회 4 (4): 577-582, 2007

13 김수미, "PLGA/DBP 필름에서 DBP 함량에 따른 슈반씨세포와 후각초성세포의 부착과 성장에 미치는 영향" 한국조직공학과 재생의학회 4 (4): 179-185, 2007

14 G Khang, "Natural scaffolds biomaterials for tissue regeneration" 1 : 9-, 2004

15 G Khang, "Natural scaffolds biomaterials for tissue regeneration" 1 : 9-, 2004

16 Jun Jae Lee, "Investigation on biodegradable PLGA scaffold with various pore size structure for skin tissue engineering" 한국물리학회 7 (7): 37-40, 2007

17 E Hedbom, "Interaction of a 59-kDa connective tissue matrix protein with collagen I and collagen II" 264 : 6898-, 1989

18 L Lu, "In vitro and in vivo defradation of porous poly(-lactic-co-glycolic acid) foams" 21 : 1837-, 2000

19 G Khang, "Improvement of wetting property for pouous PLGA scaffold by physicochemical treatment" 23 : 3-, 1999

20 Y Hu, "Fabricaion of poly(- hydroxy acid) foam scaffolds using multiple solvent systems" 59 : 563-, 2002

21 SH Kim, "Evaluation of various types of scaffold for tissue engineered intervertebral disc" 585 : 167-, 2006

22 고연경, "DBP/PLGA 하이브리드 담체를 이용한 조직공학적 바이오 디스크 개발" 한국고분자학회 31 (31): 14-19, 2007

23 PX Ma, "Biodegradable polymer scaffolds with welldefined interconnected spherical pore network" 7 : 23-, 2001

24 E Hedbom, "Binding of fibromodium and decorin to separate sites on fibrillar collagens" 268 : 27307-, 1993

25 JA Buckwater, "Aging and degeneration of the human intervertebral disc" 20 : 1307-, 1995