Abstract:It was isolated marine bacterium, isolate YJ-1 which produced a bactericidal antibiotics against MRSA. This isolate was identified as tested by examining its morphological and biochemical properties, and 16S rDNA sequencing analysis for ident...
Abstract:It was isolated marine bacterium, isolate YJ-1 which produced a bactericidal antibiotics against MRSA. This isolate was identified as tested by examining its morphological and biochemical properties, and 16S rDNA sequencing analysis for identification. This isolate YJ-1 was identified to the genus Pseudomonas. Therefore, this isolate was designated Pseudomonas sp. YJ-1. Pseudomonas sp. YJ-1. relatively grows well in the 25℃, pH 7.0, NaCl 1.0 %. For the purification of the bioactive compounds, YJ-1 was fermented in 37 L-PPES-Ⅱ medium, and culture filtrates of YJ-1 was extracted by ethyl acetate, hexane, and 80% MeOH. The 80% MeOH fraction of YJ-1 was showed the significant anti-MRSA (methicillin-resistant Staphylococcus aureus) activity against MRSA.