Adiponectin과 Leptin이 전립선암 세포의 증식에 미치는 영향 = The Effects of Adiponectin and Leptin in the Proliferation of Prostate Cancer Cells

다국어 초록 (Multilingual Abstract)

Purpose: With the westernization of dietary life, domestic prostate cancer prevalence has remarkably increased recently. Therefore, to examine the effects of obesity on prostate cancer, we analyzed the effects of leptin and adiponectin, which are the cytokines secreted from adipocytes, on prostate cancer in vitro and confirmed the results by in vivo experiment.
Materials and Methods: In vitro, the human androgen-independent prostate cancer cell line DU-145 was exposed to various concentrations of adiponectin and leptin, and their effects were measured with the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In vivo, the effects of tumor growth were observed in xenografted nude mice with prostate cancer.
Results: Adiponectin significantly repressed DU-145 cell growth in a dose- dependent manner. Leptin promoted DU-145 cell growth in dose-dependent manner, but it was not significant statistically. In vivo, adiponectin- treated mice demonstrated a reduced tumor volume, although it was not significant statistically. By contrast, leptin-treated mice showed a significantly increased tumor volume (p＜0.01).
Conclusions: The in vitro and in vivo finding suggested that adiponectin suppresses the proliferation of prostate cancer cells and that leptin plays an important role in the proliferation of prostate cancer cells. We suggest that adiponectin and leptin have a relation to the progression of prostate cancer in the obese population.

다국어 초록 (Multilingual Abstract)

Purpose: With the westernization of dietary life, domestic prostate cancer prevalence has remarkably increased recently. Therefore, to examine the effects of obesity on prostate cancer, we analyzed the effects of leptin and adiponectin, which are the cytokines secreted from adipocytes, on prostate cancer in vitro and confirmed the results by in vivo experiment.
Materials and Methods: In vitro, the human androgen-independent prostate cancer cell line DU-145 was exposed to various concentrations of adiponectin and leptin, and their effects were measured with the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In vivo, the effects of tumor growth were observed in xenografted nude mice with prostate cancer.
Results: Adiponectin significantly repressed DU-145 cell growth in a dose- dependent manner. Leptin promoted DU-145 cell growth in dose-dependent manner, but it was not significant statistically. In vivo, adiponectin- treated mice demonstrated a reduced tumor volume, although it was not significant statistically. By contrast, leptin-treated mice showed a significantly increased tumor volume (p＜0.01).
Conclusions: The in vitro and in vivo finding suggested that adiponectin suppresses the proliferation of prostate cancer cells and that leptin plays an important role in the proliferation of prostate cancer cells. We suggest that adiponectin and leptin have a relation to the progression of prostate cancer in the obese population.

참고문헌 (Reference)

1 송강현, "전립선암 환자의 진단 시기에 따른 임상적 요소들의 변화" 대한비뇨기과학회 48 (48): 574-578, 2007

2 Ribeiro R, "The link between obesity and prostate cancer: the leptin pathway and therapeutic perspectives" 9 : 19-24, 2006

3 Nazian SJ, "Temporal relation between leptin and various indices of sexual maturation in the male rat" 20 : 487-491, 1999

4 Considine RV, "Serum immunoreactive-leptin concentrations in normal-weight and obese humans" 334 : 292-295, 1996

5 Michalakis K, "Serum adiponectin concentrations and tissue expression of adiponectin receptors are reduced in patients with prostate cancer: a case control study" 16 : 308-313, 2007

6 Kissebah AH, "Quantitative trait loci on chromosomes 3 and 17 influence phenotypes of the metabolic syndrome" 97 : 14478-14483, 2000

7 Onuma M, "Prostate cancer cell-adipocyte interaction: leptin mediates androgen-independent prostate cancer cell proliferation through c-Jun NH2- terminal kinase" 278 : 42660-42667, 2003

8 Somasundar P, "Prostate cancer cell proliferation is influenced by leptin" 118 : 71-82, 2004

9 Goktas S, "Prostate cancer and adiponectin" 65 : 1168-1172, 2005

10 Wang XJ, "Potential involvement of leptin in carcinogenesis of hepatocellular carcinoma" 10 : 2478-2481, 2004

11 Zhang Y, "Positional cloning of the mouse obese gene and its human homologue" 372 : 425-432, 1994

12 Arita Y, "Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity" 257 : 79-83, 1999

13 Baillargeon J, "Obesity, adipokines, and prostate cancer in a prospective population-based study" 15 : 1331-1335, 2006

14 Carroll KK, "Obesity as a risk factor for certain types of cancer" 33 : 1055-1059, 1998

15 Chang S, "Leptin and prostate cancer" 46 : 62-67, 2001

16 Mistry T, "Leptin and adiponectin interact in the regulation of prostate cancer cell growth via modulation of p53 and bcl-2 expression" 101 : 1317-1322, 2008

17 Blum WF, "Human and clinical perspectives on leptin" 57 : 477-485, 1998

18 Cong L, "Human adiponectin inhibits cell growth and induces apoptosis in human endometrial carcinoma cells, HEC-1-A and RL95 2" 14 : 713-720, 2007

19 Takahashi M, "Genomic structure and mutations in adipose-specific gene, adiponectin" 24 : 861-868, 2000

20 Cao Y, "Expression of angiostatin cDNA in a murine fibrosarcoma suppresses primary tumor growth and produces long-term dormancy of metastases" 101 : 1055-1063, 1998

21 Yoneda K, "Expression of adiponectin receptors, AdipoR1 and AdipoR2, in normal colon epithelium and colon cancer tissue" 20 : 479-483, 2008

22 Kubota N, "Disruption of adiponectin causes insulin resistance and neointimal formation" 277 : 25863-25866, 2002

23 Kershaw EE, "Adipose tissue as an endocrine organ" 89 : 2548-2556, 2004

24 Okamoto Y, "Adiponectin reduces atherosclerosis in apolipoprotein E-deficient mice" 106 : 2767-2770, 2002

25 Dieudonne MN, "Adiponectin mediates antiproliferative and apoptotic responses in human MCF7 breast cancer cells" 345 : 271-279, 2006

26 Bub JD, "Adiponectin as a growth inhibitor in prostate cancer cells" 340 : 1158-1166, 2006

27 Bub JD, "Adiponectin as a growth inhibitor in prostate cancer cells" 340 : 1158-1166, 2006

28 Miyazaki T, "Adiponectin activates c-Jun NH2-terminal kinase and inhibits signal transducer and activator of transcription 3" 333 : 79-87, 2005

29 Barb D, "Adioponectin in relation to malignancies: a review of existing basic research and clinical evidence" 86 : s858-s866, 2007