목적: 본 연구는 난소암세포주 SK-OV-3세포에서 마이토콘드리아 단백질인 p32가 pro-apoptotic 단백질인 Harakiri와 관계된 세포사멸에 관한 생리적 기능을 알아보고자 하는데 목적이 있다. 방법: p32...
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https://www.riss.kr/link?id=A75054340
2008
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세포사멸 ; 난소암세포 ; p32 ; Harakiri ; Apoptosis ; Cell death ; Ovarian cancer
500
KCI등재,SCOPUS,ESCI
학술저널
858-865(8쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
목적: 본 연구는 난소암세포주 SK-OV-3세포에서 마이토콘드리아 단백질인 p32가 pro-apoptotic 단백질인 Harakiri와 관계된 세포사멸에 관한 생리적 기능을 알아보고자 하는데 목적이 있다. 방법: p32...
목적: 본 연구는 난소암세포주 SK-OV-3세포에서 마이토콘드리아 단백질인 p32가 pro-apoptotic 단백질인 Harakiri와 관계된 세포사멸에 관한 생리적 기능을 알아보고자 하는데 목적이 있다. 방법: p32와 Harakiri의 immunoprecipitation을 통해 상호작용을 확인하였으며, confocal microscopy를 이용하여 면역형광염색을 통해 세포 내 위치를 확인하였다. 난소암세포주인 SK-OV-3세포에서 p32와 Harakiri를 과발현시켜 36시간 후에 형광현미경에 서 GFP가 발현하는 세포를 counting하였다. 결과: p32와 Harakiri는 난소암세포주에서 세포사멸을 일으켰으며, p32와 Harakiri가 세포질 내 마이토콘드리아에 함께 존재함으로써 그 기능을 수행하며 상호작용하고 있음을 알았다. 또한, p32와 Harakiri가 동시에 과발현시켰을 때 세포사멸의 효과가 더 강력함을 알았다. 결론: 본 연구를 통해 난소암세포에서 p32가 새로운 세포사멸에 유도체임을 밝히게 되었으며, p32는 난소암세포 치료제로서 응용도 가능하다고 짐작해 본다.
다국어 초록 (Multilingual Abstract)
Objective: The purpose of the study was to examine a possible physiological function of p32-mediated apoptosis signaling in ovarian cancer cells. Methods: SK-OV-3 cells were transfected with respective plasmid DNAs, and cell viability was measured. By...
Objective: The purpose of the study was to examine a possible physiological function of p32-mediated apoptosis signaling in ovarian cancer cells. Methods: SK-OV-3 cells were transfected with respective plasmid DNAs, and cell viability was measured. By immunoprecipitation and immunofluorescence staining analysis, we confirmed that p32 interacts with Harakiri in ovarian cancer cells. Results: In SK-OV-3 cells, p32 interacted with Harakiri and both p32 and Harakiri were colocalized in the mitochondria. In addition, overexpression of p32 induced apoptosis of ovarian cancer cells and augmented Harakiri-mediated apoptosis. Conclusion: Our results demonstrated p32 as an apoptosis inducer and helped to provide the better understanding of the function of p32 in ovarian cancer cells and a possibility of p32 in the application of cancer therapeutics
참고문헌 (Reference)
1 Ma C, "dp5/HRK is a c-Jun target gene and required for apoptosis induced by potassium deprivation in cerebellar granule neurons" 282 : 30901-30909, 2007
2 Kamal A, "Upregulation of hyaluronan binding protein 1 (HABP1/p32/gC1qR) is associated with Cisplatin induced apoptosis" 11 : 861-874, 2006
3 Knudson W, "The role and regulation of tumor associated hyaluronan" 143 : 150-159, 1989
4 Ghobrial IM, "Targeting apoptosis pathways in cancer therapy" 55 : 178-194, 2005
5 Lin J, "Suppression activity of pro-apoptotic gene products in cancer cells, a potential application for cancer gene therapy" 21 : 831-839, 2001
6 안윤걸, "SK-OV-3 상피성 난소암세포주에서 선택적 COX-2 억제제인 celecoxib가 paclitaxel에 의한 세포자멸사에 미치는 영향" 대한산부인과학회 48 (48): 1673-1685, 2005
7 Rubinstein DB, "Receptor for the globular heads of C1q (gC1q-R, p33, hyaluronan-binding protein) is preferentially expressed by adenocarcinoma cells" 110 : 741-750, 2004
8 Sunayama J, "Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32" 11 : 771-781, 2004
9 Imaizumi K, "Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death" 272 : 18842-18848, 1997
10 Certo M, "Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members" 9 : 351-365, 2006
1 Ma C, "dp5/HRK is a c-Jun target gene and required for apoptosis induced by potassium deprivation in cerebellar granule neurons" 282 : 30901-30909, 2007
2 Kamal A, "Upregulation of hyaluronan binding protein 1 (HABP1/p32/gC1qR) is associated with Cisplatin induced apoptosis" 11 : 861-874, 2006
3 Knudson W, "The role and regulation of tumor associated hyaluronan" 143 : 150-159, 1989
4 Ghobrial IM, "Targeting apoptosis pathways in cancer therapy" 55 : 178-194, 2005
5 Lin J, "Suppression activity of pro-apoptotic gene products in cancer cells, a potential application for cancer gene therapy" 21 : 831-839, 2001
6 안윤걸, "SK-OV-3 상피성 난소암세포주에서 선택적 COX-2 억제제인 celecoxib가 paclitaxel에 의한 세포자멸사에 미치는 영향" 대한산부인과학회 48 (48): 1673-1685, 2005
7 Rubinstein DB, "Receptor for the globular heads of C1q (gC1q-R, p33, hyaluronan-binding protein) is preferentially expressed by adenocarcinoma cells" 110 : 741-750, 2004
8 Sunayama J, "Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32" 11 : 771-781, 2004
9 Imaizumi K, "Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death" 272 : 18842-18848, 1997
10 Certo M, "Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members" 9 : 351-365, 2006
11 Willis SN, "Life in the balance: how BH3-only proteins induce apoptosis" 17 : 617-625, 2005
12 Toole BP, "Hyaluronan: from extracellular glue to pericellular cue" 4 : 528-539, 2004
13 Knudson CB, "Hyaluronan-binding proteins in development, tissue homeostasis, and disease" 7 : 1233-1241, 1993
14 Laurent TC, "Hyaluronan" 6 : 2397-2404, 1992
15 Inohara N, "Harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)" 16 : 1686-1694, 1997
16 Reed, JC, "Dysregulation of apoptosis in cancer" 17 : 2941-2953, 1999
17 Ghosh I, "Differential expression of Hyaluronic Acid Binding Protein 1 (HABP1)/P32/C1QBP during progression of epidermal carcinoma" 267 : 133-139, 2004
18 Imaizumi K, "Critical role for DP5/Harakiri, a Bcl-2 homology domain 3-only Bcl-2 family member, in axotomy-induced neuronal cell death" 24 : 3721-3725, 2004
19 Harris CA, "BH3-only Bcl-2 family members are coordinately regulated by the JNK pathway and require Bax to induce apoptosis in neurons" 276 : 37754-37760, 2001
20 Matthews DA, "Adenovirus core protein V interacts with p32--a protein which is associated with both the mitochondria and the nucleus" 79 : 1677-1685, 1998
임신 초기 시행한 질식초음파의 전치태반 예측에 대한 연구
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2013-01-10 | 학술지명변경 | 한글명 : Korean Journal of Obstetrics and Gynecology -> Obstetrics & Gynecology Science외국어명 : Korean Journal of Obstetrics and Gynecology -> Obstetrics & Gynecology Science | |
2013-01-01 | 평가 | 등재 1차 FAIL (등재유지) | |
2011-01-15 | 학술지명변경 | 한글명 : 대한산부인과학회지 -> Korean Journal of Obstetrics and Gynecology | |
2010-06-14 | 학술지명변경 | 한글명 : 대한산부인과학회잡지 -> 대한산부인과학회지 | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2006-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2005-05-24 | 학회명변경 | 영문명 : 미등록 -> Korean Soceity of Obstetrics and Gynecology | |
2005-03-22 | 학술지등록 | 한글명 : 대한산부인과학회잡지외국어명 : Korean Journal of Obstetrics and Gynecology | |
2005-01-01 | 평가 | 등재후보학술지 유지 (등재후보2차) | |
2004-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2002-01-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.04 | 0.04 | 0.06 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.06 | 0.06 | 0.255 | 0 |