녹용의 약효 성분에 관한 연구 ( 3 ) 녹용 및 Pantocrin 의 지방산 조성에 관하여 = Biochemical Studies on Antler ( Cervus nippon taiouanus ) ( 3 ) A Study of Free and Ester Fatty Acids of Antler Velvet Layer and Pantocrin

다국어 초록 (Multilingual Abstract)

The lipid soluble fraction of antler velvet layer (Cervus nippon taiouanus) was extracted and compared to that of pantocrin (ethanol preparation of antler, commericially available). The lipid soluble components(801.5㎎ from antler velvet layer and 979.1㎎/150㎖ from pantocrin) were fractionated into neutral lipids, glycolipids, phosphclipids, gangliosides, and nonlipid components (lipoprotein and peptide) on Sephadex G-25 column followed by silicic acid column chromatography. The percentage composition of the lipid soluble fraction was neutral lipid 65.3%, glycolipid 5.2%, phospholipid 12.9%, ganglioside 6.1 %, nolipid 2.9 %. The neutral lipids were fractionated into squalene, sterolester, triglyceride, free fatty acid, sterol, diglyceride, monoglyceride on boric acid impregnated silicic acid column. The fractions collected were determined by thin layer chromatography. The compositions of the free fatty acid fractions were determined by gas liquid chromatography. The sterolester, triglyceride fractions were hydrolyzed to give free fatty acids with 10% KOH solution, refluxed at 80-85℃ for four hours, and carried out gas liquid chromatography. The free fatty acids in antler velvet layer were found to be nine kinds of saturated fatty acids, nine kinds of unsaturated fatty acids, two branched fatty acids and six unknown components. The main components of antler free fatty sacid were oleic (21.7%), linolenic(31.2%), palmitic(19.6%), stearic(12.1%), palmitoleic(10.2%), and those make 94.4% of antler free fatty acids. In pantocrin free fatty acid fraction, seven kinds of saturated fatty acids, four kinds of unsaturated fatty acids, one branched fatty acid and three unknown compopents were detected. The main components of pantocrin free fatty, acid fraction wereoleic(45%), palmitic(19.5%), lauric(10.9%), stearic(6.6%), palmitoleic(5.6%), myristic(3.4%), capric(3.1%), and those make 94.1% of pantocrin free tfatty acids. The ester fatty acids of triglyceride fraction were found as follows: In antler triglyceride faction, nine kinds of saturated fatty acids, three kinds of unsaturated fatty acids, three branched fatty acids and ten unknown components were detected. In pantocrin triglyceride fraction, it was found to contain five kinds of saturated fatty acids, two kinds of unsaturated fatty acids, one branched fatty acid and ten unknown components. after saponification of triglyceride fraction, two new fatty acids (pentadecylic, oleic) in antler neutral lipid and two new fatty acids (lauric, dodecenoic) in pantocrin neutral lipid were detected. In sterol ester fraction, there were six saturated fatty acids, three unsaturated fatty acids, one branched fatty acid and eight unknown components in antler neutral lipid, and five saturated fatty acid, three unsaturated fatty acids and five unknown components in pantocrin neutral lipid. After saponification of sterol ester fractions, new fatty acids were determined as dodecenoic, lauric, anteiso-pentadecylic, isopalmitic, heptadecanoic in antler sterol ester fraction and dodecenoic, palmitic, heptadecanoic, heptadecenoic in pantccrin sterol ester fraction.