Pheophytin Content and Cytotoxicity of Silkworm Feces Against Jurkat Cells According to Dry Method and Storage Period

다국어 초록 (Multilingual Abstract)

In order to find potential anticancer agents, we
extracted pheophytin from silkworm feces according
to various dry and storage methods such as sun dry,
shade dry, fresh freezing dry and freezing dry after
freezing storage (for 1 ~ 3 years). The pheophytin
extracts, mainly 10-hydroxypheophytin a, little b, of
various storage silkworm feces were analyzed by
reversed-phase high-performance liquid chromatography
with photodiode array and fluorescence detection.
The content of those pheophytin in old silkworm for 3
years (freezing storage and freezing dried in use, or
freezing dried and cold storage) was better than others.
The cytotoxicity of the pheophytin extracts and
ethanol extracts of various storage silkworm feces
were measured using Jurkat cells originated from
human leukemia, using dye uptake assay (MTT) in
order to find effective photodynamic therapeutic
agents. The anticancer activity of those pheophytin
extracts in various storage methods showed little difference
among them. But ethanol extracts of fresh
freezing dried silkworm in the current year was good
cytotoxic activity than those of any other silkworm
feces. With regards to these results, fresh ethanol
extracts of silkworm feces were better than old ones.
On the other hands, the pheophytin extracts of old
silkworm feces contained the highest percentage of
pheophytin content and showed good cytotoxicity
against cancer cells by changing the pheophytin into
pheophobide in the degradative process.

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