능이 [Sarcodon aspratus (Berk.) S. Ito]로부터 蛋白質加水分解 酵素를 분리 정제하여 그 특성을 조사하였다.<br/> 본 효소는 Tri-acryl CM-cellulose, Ultrogel AcA 54, Hydroxy apatite column chromatography와 preparati...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A76056621
1986
Korean
SCOPUS,KCI등재
학술저널
276-285(10쪽)
0
상세조회0
다운로드국문 초록 (Abstract)
능이 [Sarcodon aspratus (Berk.) S. Ito]로부터 蛋白質加水分解 酵素를 분리 정제하여 그 특성을 조사하였다.<br/> 본 효소는 Tri-acryl CM-cellulose, Ultrogel AcA 54, Hydroxy apatite column chromatography와 preparati...
능이 [Sarcodon aspratus (Berk.) S. Ito]로부터 蛋白質加水分解 酵素를 분리 정제하여 그 특성을 조사하였다.<br/>
본 효소는 Tri-acryl CM-cellulose, Ultrogel AcA 54, Hydroxy apatite column chromatography와 preparative isoelectric focusing 방법으로 순차 정제되었고, 정제된 효소는 PAGE상에서 단일 band를 나타내었으며 活性은 62.5O, D/㎎, protein으로 粗酵素보다 8.01배 증가하였다.<br/>
作用最適 pH는 10.1로 alkaline protease였으며 作用最適 溫度는 57℃부근이었다.<br/>
50℃ 이하의 溫度와 pH 4.0~10.8 범위에서 安定하였으나, 60℃에서 30분후 26℃, 65℃에서는 65℃가 失活되었다.<br/>
Mn^(++)은 효소의 活性을 증가시켰으나 Hg^(++)와 Cu^(++)는 현저히 저해시켰다.<br/>
SDS-PAGE에 의해 分子量은 30,100으로 측정되었고, monomer임이 확인되었다.<br/>
等電點은 9.80이었다.<br/>
본 연구는 1985년 문교부 학술연구조성비에 의해 연구되었읍니다.
다국어 초록 (Multilingual Abstract)
This study was undertaken to investigate the characteristics of the proteolytic enzyme extracted from Neungee mushroom [Sarcodon aspratus (Berk.) S. Ito].<br/> The enzyme was purified by using Tris-acryl CM-cellulose ion exchange, gel filtratio...
This study was undertaken to investigate the characteristics of the proteolytic enzyme extracted from Neungee mushroom [Sarcodon aspratus (Berk.) S. Ito].<br/>
The enzyme was purified by using Tris-acryl CM-cellulose ion exchange, gel filtration on Ultrogel AcA 54, Hydroxy apatite column chromatography and preparative isoelectic focusing.<br/>
The specific activity of the purified enzyme increased 8 times as compared with that of the crude enzyme. The enzyme was homogeneous on polyacrylamide gel electrophoresis (PAGE).<br/>
The optimum pH was 10.1, indicating the enzyme to be alkaline protease and the optimum temperature was 57℃.<br/>
The enzyme was stable at temperatures lower than 50℃ and at pH values ranging from 4.0 to 10.8.<br/>
However, the enzyme activity decreased by 26 and 65% at 60 and 65℃, respectively, when incubated for 30 minutes.<br/>
The enzyme activity was activated by Mn^(++) and inhibited by Cu^(++) and Hg^(++).<br/>
The enzyme was consisted of monomer and its molecular weight estimated to be about 30,100 when determined by sodium dodecyl sulfate PAGE.<br/>
Isoelectric point of the enzyme was determined to be 9.80.
잉어육과 잉어자숙(煮熟)중의 아미노산 및 지방산조성의 변화