The stacking sodium dodecylsulfate-acrylamide gel electrophoresis method was used to determine the non-meat protein content in chicken products. Densitograms of the selected protein band, denoted by SI, MI, used for the quantitative estimation of isol...
The stacking sodium dodecylsulfate-acrylamide gel electrophoresis method was used to determine the non-meat protein content in chicken products. Densitograms of the selected protein band, denoted by SI, MI, used for the quantitative estimation of isolated soy protein (ISP) and milk protein (MP), respectively. The equation of the line of best fit fot peak area ratio (y) versus protein concentration ratio (X) of ISP/ chicken protein was found to be ; y = 1.5096X + 0.0163 (r=0.9318). And for MP content the corresponding line of best fit was y=1.3536X - 0.0112 (r = 0.9693). These protein content could be determined within 2% of the true value in the range of 10∼30% ISP and MP respectively.