The present experiment was investigated on the effects of SaGunJaTang extract(SGJex) on the sarcoplasmic reticulum ATPase activity.
The result demonstrated that the ATPase activity of sarcoplasmic reticulum was activated in the presence of SaGunJa ex...
The present experiment was investigated on the effects of SaGunJaTang extract(SGJex) on the sarcoplasmic reticulum ATPase activity.
The result demonstrated that the ATPase activity of sarcoplasmic reticulum was activated in the presence of SaGunJa extract, the activation effect of the extract was analyzed with time course, Lineweaver -Burk plot, and Arrhenius plot. The activation mechanism of SGJex on the enzyme was proposed by increasing the affinity between active site of the enzyme and ATP prolecules, and the energy of activation was decreased in the presence of SaGunJa extract.
The results were obtained as follows :
1. The sarcoplasmic reticulum ATPase was free from the mitochondrial contamination in the present experiment, the optimum pH was 6.8 and the optimum concentrations of Ca^++, Mg^++ and SaGunJa extract were 0.1mM 4mM and 1% respectively.
2. The Km value of the enzyme was 7.81, however the Km values effected by SaGunJa extracts of 0.1% and 1% were decreased to 5.44 and 3.85 respectively.
3. The energy of activation of the control was 16.2 ㎉/mole, and the values were decreased to 15.4 ㎉/mole and 14.88 ㎉/mole in the presence of 0.1% and 1% SGJex respectively.