Isolation, Physicochemical Properties and Toxicities of Territrem A' and B'

다국어 초록 (Multilingual Abstract)

We have isolated two new metabolites of territrem, designated as territrem A`^* (TRA`; C_(28)H_(30)O_(10) and B`(TRB`; C₂₉H₃₄O_(10)) from chloroform extract of rice culture of Aspergillus terreus 23-1, using the same isolation procedure as that for territrem A, B and C(TRA, TRB, TRC). The present isolation procedure gave about 5 ㎎ of TRA` and 10 ㎎ of TRB` from 4 ㎏ of rice culture per batch. Analysis of the high resolution mass spectrum showed that the molecular composition of TRA` and TRB` are C_(28)H_(30)O_(10) and C₂₉H₃₄O_(10) respectively, Some results of physicochemical properties were presented in this paper. Single crystal X-ray diffractometry of TRB` showed that the three dimensional structure of TRB` has not changed significantly from that of TRB (C₂₉H₃₄O₉), except for the insertion of one oxygen atom into TRB to make additional pyran in the E-ring. It is also suggested that the aromatic moiety of TRA` is similar to that of TRA (C_(28)H_(30)O<sub>9</sub>) and the rest non-aromatic portions resemble to those of TRB`. The tremorgenic activity, lethality and inhibitory effect on acetylcholine esterase of TRA` and TRB` are greatly reduced comparing to that of TRA and TRB.