Cloning and Characterization of the UDP Glucose/Galactose Epimerases of Oryza sativa = Cloning and Characterization of the UDP Glucose/Galactose Epimerases of Oryza sativa

다국어 초록 (Multilingual Abstract)

UDP-glucose/galactose-4-epimerase (UGE) catalyzes the interconversion of UDP-glucose and UDP-galactose. Oryza sativa contains four UGEs. Expression of OsUGE3 was higher than other OsUGEs in all tissues except the stem. OsUGE1 expression was enhanced after drought, salt or UV-irradiation stress. Four rice UGEs (OsUGE1-4) were cloned by polymerase chain reaction and sequenced. OsUGE1-4 were expressed in Escherichia coil with glutathione S-transferase fusion protein and purified. All four OsUGEs could covert UDP-glucose into UDP-galactose better than vice versa. Kinetic parameters of OsUGEs showed that OsUGE1 was most efficient. These OsUGEs could be used for biosynthesis of various UDP-sugars, which serve as a cosubstrates of UDP-dependent glycosyltransferase modification reactions of antibiotics or flavonoids.

참고문헌 (Reference)

1 Rösti J, "UDP-glucose 4-epimerase isoforms UGE2 and UGE4 cooperate in providing UDP-galactose for cell wall biosynthesis and growth of Arabidopsis thaliana" Plant Cell 19 : 1565-1579, 2007

2 Hollman P, "The sugar moiety is a major determinant of the absorption of dietary flavonoid glycosides in man" Free Radic Re 31 : 569-573, 1999

3 Dörmann P, "The role of UDP-glucose epimerase in carbohydrate metabolism of Arabidopsis" Plant J 13 : 641-652, 1998

4 Burget EG, "The mur4 mutant of Arabidopsis is partially defective in the de Novo synthesis of uridine diphospho L-arabinose" Plant Physiol 121 : 383-389, 1999

5 Burget EG, "The biosynthesis of L-arabinose in plants: Molecular cloning and characterization of a Golgi-localized UDPD-xylose 4-epimerase encoded by the MUR4 gene of Arabidopsis" Plant Cell 15 : 523-531, 2003

6 Gu X, "The Biosynthesis of UDPgalacturonic acid in plants. Functional cloning and charaterizationn of Arabidopsis UDP-D-glucuronic acid 4-epimerase" Plant Physiol 136 : 4256-4264, 2004

7 Endo N, "Putative UDP-galactose epimerase and metallothineine of Paspalum vaginatum enhanced the salt tolerance of rice, Oryza sativa L. from transplanting to harvest stages" Breeding Sci 55 : 163-173, 2005

8 Weirenga RK, "Prediction of the occurrence of the ADP-binding beta alpha betafold in proteins, using an amino acid sequence fingerprint" J Mol Biol 187 : 101-107, 1986

9 Liu H, "Over-expression of OsUGE-1 altered raffinose level and tolerance to abiotic stress but not morphology in Arabidopsis" J Plant Physiol 164 : 1384-1390, 2007

10 Seifert GJ, "Nucleotide sugar interconversionns and cell wall biosynthesis: how to bring the inside to the outside" Cur Opin in Plant Biol 7 : 277-284, 2004

11 Rabbani MA, "Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses" Plant Physiol 133 : 1755-1767, 2003

12 Reiter WD, "Molecular genetics of nucleotide sugar interconversion pathways in plants" Plant Mol Biol 47 : 95-113, 2001

13 Vogt T, "Glycosyltransferases in plant natural product synthesis: characterization of a supergene family" Trends Plant Sci 5 : 380-386, 2005

14 Zhang, Q, "Gene expression patterns and catalytic properties of UDP-D-glucose 4-epimerase from barley (Hordeum vulgare L.)" Biochem J 394 : 115-124, 2006

15 Dörmann P, "Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli" Arch Biochem Biophys 327 : 27-34, 1996

16 Bong-Gyu Kim, "Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization" 한국식물학회 52 (52): 41-48, 2009

17 Barber C, "Distinct properties of the five UDP-Dglucose/UDP-D-galactose 4-epimerase isoforms of Arabidopsis thaliana" J Biol Chem 281 : 17276-17285, 2006

18 Suzuki K, "Cloning and expression of a UDP-glucuronic acid decarboxylase gene in rice" J Exp Bot 54 : 1997-1999, 2003

19 Pridham JB, "Biosynthesis of Raffinose" Plant Physiol 40 : 984-986, 1965

20 Bong Gyu Kim, "Anthocyanin Content in Rice Is Related to Expression Levels of Anthocyanin Biosynthetic Genes" 한국식물학회 50 (50): 156-10, 2007

21 Egelund J, "A complementary bioinformatics approach to identify potential plant cell wall glycosyltransferaseencoding genes" Plant Physiol 136 : 2609-2620, 2004