Impact of Zinc Stress on Biochemical and Biophysical Parameters in Coffea Arabica Seedlings

다국어 초록 (Multilingual Abstract)

Zinc is an essential micronutrient for the healthy development of plants, since its insufficient and supraoptimal doses can disrupt the metabolism and biomass production. We aimed to investigate the physiological responses of coffee seedlings to Zn deficiency and excess. Six-month-old seedlings were transferred to plastic pots containing a nutrient solution. The treatments were control (0.03 ppm), zinc deficiency (0.00 ppm), and zinc excess (0.12 ppm). The evaluations were performed in leaves and roots at the beginning of the treatments and after 30 and 60 d of treatments. Zn deficiency and excess increased the production of hydrogen peroxide, antioxidant enzymes activity, ascorbate, and lipid peroxidation contents. The imbalance in zinc nutrition reduced total chlorophyll content and increased carotenoids content throughout the experimental period. Lower biomass and proline accumulation were observed only for deficient seedlings at the end of the experiment. The characteristics analyzed showed that zinc deficiency caused greater damage to the Coffea arabica plants of (Catuai cultivar) than zinc excess.

참고문헌 (Reference)

1 Vassilev AA, "effects of excess zn on growth and photosynthetic performance of young bean plants" 3 : 58-62, 2011

2 Lichtenthaler HK, "chlorophylls and carotenoids:measurement and characterization by uv-vis spectroscopy" 1 (1): F4.3.1-F4.3.8, 2001

3 Andrade SAL, "Zn uptake, physiological response and stress attenuation in mycorrhizal jack bean growing in soil with increasing Zn concentrations" 75 : 1363-1370, 2009

4 Li X, "Zinc-induced oxidative damage, antioxidant enzyme response and proline metabolism in roots and leaves of wheat plants" 89 : 150-157, 2013

5 Aravind P, "Zinc protects chloroplasts and associated photochemical functions in cadmium exposed Ceratophyllum demersum L., a freshwater macrophyte" 166 (166): 1321-1327, 2004

6 Caldelas C, "Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc" 62 (62): 2169-2178, 2010

7 Feigl G, "Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species(ROS and RNS)in the roots of two Brassica species with different sensitivity to zinc stress" 116 (116): 613-625, 2014

8 Höller S, "Zinc deficiency differentially affects redox homeostasis of rice genotypes contrasting in ascorbate level" 171 (171): 1748-1756, 2014

9 Mattiello EM, "Zinc deficiency affects physiological and anatomical characteristics in maize leaves" 183 : 138-143, 2015

10 Hoagland DR, "The water culture method for growing plants without soil" 347 : 1-32, 1950

11 Sharma SS, "The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress" 57 (57): 711-726, 2006

12 Noctor G, "The roles of reactive Oxygen metabolism in drought : Not so cut and dried" 164 (164): 1636-1648, 2014

13 Nazarbeygi E, "The effects of different levels of salinity on proline and A-, B-chlorophylls in Canola" 10 : 70-74, 2011

14 Michael PI, "The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings" 74 : 171-177, 2011

15 Ozdener Y, "The effect of zinc on the growth and physiological and biochemical parameters in seedlings of Eruca sativa (L.) (Rocket)" 32 (32): 469-476, 2009

16 Cakmak, I, "Tansley Review No. 111 Possible roles of zinc in protecting plant cells from damage by reactive oxygen species" 146 (146): 185-205, 2000

17 Giannopolitis CN, "Superoxide Dismutases: I. Occurrence in higher plants" 59 (59): 309-314, 1997

18 Hashemi SA, "Studying the effects of heavy metal on chlorophyll a nd sugar in one year-old seedlings organs of Acer velutinum specie" 38 (38): 224-227, 2018

19 Ferreira DF, "Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia" 35 (35): 1039-1042, 2011

20 Alvarez-Flores R, "Root foraging capacity depends on root system architecture and ontogeny in seedlings of three Andean Chenopodium species" 380 (380): 415-428, 2014

21 Bandurska H, "Regulation of proline biosynthesis and resistance to drought stress in two barley (Hordeum vulgare L.) genotypes of different origin" 118 : 427-437, 2017

22 Karuppanapandian TJC, "Reactive oxygen species in plants : their generation, signal transduction, and scavenging mechanisms" 5 (5): 709-, 2011

23 Gill SS, "Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants" 48 (48): 909-930, 2010

24 Biemelt S, "Re-aeration following hypoxia or anoxia leads to activation of the antioxidative defense system in roots of wheat seedlings" 116 (116): 651-658, 1998

25 Noctor G, "ROS-related redox regulation and signaling in plants" 80 : 3-12, 2018

26 Islam F, "Proteus mirabilis alleviates zinc toxicity by preventing oxidative stress in maize(Zea mays)plants" 110 : 143-152, 2014

27 Liang XL, "Proline mechanisms of stress survival" 19 (19): 998-1011, 2013

28 Epstein E, "Princípios e perspectivas" Planta Londrina 2006

29 Vangronsveld J, "Phytoremediation of contaminated soils and groundwater : lessons from the field" 16 (16): 765-794, 2009

30 Velikova V, "Oxidative stress and some antioxidant systems in acid raintreated bean plants : protective role of exogenous polyamines" 151 : 59-66, 2000

31 Kishor P, "Overexpression of 1-pyrroline-5-carboxylate synthetase increases proline overproduction and confers osmotolerance in transgenic plants" 108 (108): 1387-1394, 1995

32 Fu C, "Morphology, photosynthesis, and internal structure alterations in field apple leaves under hidden and acute zinc deficiency" 193 : 47-54, 2015

33 Cakmak I, "Morphological and hysiological differences in the response of cereals to zinc deficiency" 100 : 349-357, 1998

34 Jain R, "Impact of excess zinc on growth parameters, cell division, nutrient accumulation, photosynthetic pigments and oxidative stress of sugarcane (Saccharum spp.)" 32 (32): 979-986, 2010

35 Nakano Y, "Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts" 1981

36 Prasad MNV, "Heavy metal stress in plants: From Biomolecules to ecosystem" Springer- Verlag 2004

37 Rizvi A, "Heavy metal induced oxidative damage and root morphology alterations of maize (Zea mays L.) plants and stress mitigation by metal tolerant nitrogen fixing Azotobacter chroococcum" 157 : 9-20, 2018

38 Pavlíková D, "Glutamate kinase as a potential biomarker of heavy metal stress in plants" 70 (70): 223-230, 2008

39 Peever TL, "Electrolyte leakage, lipoxygenase, and lipid peroxidation induced in tomato leaf tissue by specific and nonspecific elicitors from Cladosporium fulvum" 90 (90): 867-875, 1989

40 Paunov M, "Effects of different metals on photosynthesis : Cadmium and zinc affect chlorophyll fluorescence in Durum Wheat" 19 (19): 787-, 2018

41 Chu W, "Effects of cuticular wax on the postharvest quality of blueberry fruit" 239 : 68-74, 2018

42 Torello WA, "Effects of NaCl stress on proline and cation accumulation in salt sensitive and tolerant turfgrasses" 93 (93): 241-247, 1986

43 Alia Prasad KVSK, "Effect of zinc on free radicals and proline in Brassica juncea and Cajanus cajan" 39 : 45-47, 1995

44 Rout GR, "Effect of metal toxicity on plant growth and metabolism: I" 873-884, 2009

45 Soltanghei A, "Effect of Zinc and Phosphorus supply on the activity of carbonic anhydrase and the ultrastructure of chloroplast in sweet corn (Zea mays var. saccharata)" 13 (13): 51-58, 2014

46 Jiang K, "Ecotoxicological effects of metals with different concentrations and types on the morphological and physiological performance of wheat" 167 : 345-353, 2019

47 Faquin V, "Diagnose do estado nutricional das plantas" 1 : 77-, 2002

48 Santos JO, "Copper(Cu)stress affects carbon and antioxidant metabolism in Coffea arabica seedlings" 11 (11): 960-967, 2017

49 Zabini AV, "Concentração de micronutrientes e características bioquímicas de progênies de cafeeiros (Coffea arabica L.) eficientes no uso de zinco" 23 (23): 95-103, 2007

50 Vaillant N, "Comparative study of responses in four Datura species to zinc stress" 59 (59): 1005-1013, 2005

51 Havaux M, "Carotenoid oxidation products as stress signals in plants" 79 (79): 597-606, 2013

52 Guimarães RJ, "Cafeicultura" UFLA/FAEPE 2002

53 Clemente JM, "Boron, copper, and zinc affect the productivity, cup quality, and chemical compounds in coffee beans" 201 : 1-14, 2018

54 Clemente JM, "Boron, copper and zinc effects on photosynthesis, enzymatic activity, nutritional status, production, chemical composition and cup quality of coffee" Universidade Federal de Viçosa 2014

55 Lee J-S, "Biochemical indicators of root damage in rice(Oryza sativa)genotypes under zinc deficiency stress" 130 (130): 1071-1077, 2017

56 Frei M, "Biochemical factors conferring shoot tolerance to oxidative stress in rice grown in low zinc soil" 37 (37): 74-, 2010

57 Havir EA, "Biochemical and developmental characterization of multiple forms of catalase in tobacco leaves" 84 (84): 450-455, 1987

58 Malavolta E, "Avaliação do estado nutricional das plantas:princípios e aplicações" Potafos 1997

59 Blasco B, "Antioxidant response and carboxylate metabolism in Brassica rapa exposed to different external Zn, Ca, and Mg supply" 176 : 16-24, 2015

60 Cherif J, "Analysis of in vivo chlorophyll fluorescence spectra to monitor physiological state of tomato plants growing under zinc stress" 101 (101): 332-339, 2010

61 Hildebrandt TM, "Amino acid catabolism in plants" 8 (8): 1563-1579, 2015

62 Jaleel CA, "Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits" 61 (61): 298-303, 2008

63 Arakawa N, "A Rapid and Sensitive Method for the Determination of Ascorbic Acid using 4, 7-Diphenyl-l, 10-phenanthroline" 45 (45): 1289-1290, 1981