An Investigation of the Analgesic and Anti-Inflammatory Effects of Nigella sativa Seed Polyphenols

다국어 초록 (Multilingual Abstract)

Extracts obtained from the seeds of Nigella sativaare used as a spice or remedy for the treatment of variousinflammatory diseases. The purpose of this study was to examine the analgesic and anti-inflammatory effects of its polyphe-nols. N. sativaseed polyphenols were prepared, and analgesic and anti-inflammatory effects were studied in mice and rats us-ing the acetic acid-induced writhing, formalin, light tail flick, carrageenan-induced paw edema, and croton oil-induced earedema tests. In the acetic acid-induced writhing test, oral administration of N. sativa polyphenols (NSP) decreased the num-ber of abdominal constrictions. Both oral and intraperitoneal administration of NSP significantly suppressed in a dose-de-pendent manner the nociceptive response in the early and late phases of the formalin test, and the effect on the late phase wasmore pronounced. Pretreatment with naloxone failed to reverse the analgesic activity of NSP in this test. NSP did not pro-duce a significant analgesia in the light tail flick test in mice. Oral administration of NSP did not produce a significant re-duction in carrageenan-induced paw edema. However, when injected intraperitoneally, NSP inhibited paw edema in a dose-dependent manner. NSP when applied topically failed to reduce croton oil-induced ear edema. These results suggest that NSPhave analgesic and anti-inflammatory effects. The lack of analgesic effect of NSP in the light tail flick test and also the fail-ure of naloxone to reverse the analgesia in the formalin test reveal that mechanisms other than stimulation of opioid recep-tors are involved.

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