The observations on the histological changes of muscular tissue during the processes, such as drying, freezing and thawing were carried out to get basic data for processes and storage of puffer, Fugu xanthopterus.
The results are as follows :
Epid...
The observations on the histological changes of muscular tissue during the processes, such as drying, freezing and thawing were carried out to get basic data for processes and storage of puffer, Fugu xanthopterus.
The results are as follows :
Epidermal part is tough and thick. That is connected with muscular tissue by soft and tough connective tissue .
The muscular tissue structures of puffer are epidermis, corium and partially dark muscle, without hypodermic fat.
According to the respective muscular parts, there exist crossed muscle fibers like abalone.
The connective tissues of intercellular materials and myocommata were found abundantly.
During the couresc of drying, the dehydration of muscular tissue occured on connective tissue in the early stage ; thus caused widening between muscle bundles respectively. In the late stage of drying, contraction of myocommata and the widening between muscle bundles were appeared.
After frozen storage for a month, distributions of ice crystal were affected deeply by freezing temperature, that is. those were observed mainly on the intracellular sites when frozen at -35℃. while on the extracellular sites when frozen at -10℃. but the ice crystals formed in both temperatures caused the condensation of muscle fibers.
Thawing fter frozen storage for a month, a recovery of muscle fibers and figuration were observed in muscular tissue stored at -35℃, while insufficien recovery of muscle fibers and disfiguration by dehydration at -10℃.
In the observation of uscle figbers when the tissue was homogenated with 1% cold formali. the fresh tissue was fragmented to myofibril level and intermixed like thred, while the frozen tissue for a month became rigid due to disfiguration, contraction, the loss of elasticity and water holding capacity.