Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414

다국어 초록 (Multilingual Abstract)

A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU).
Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.

참고문헌 (Reference)

1 Michetti, P., "Vaccine against Helicobacter pylori: fact or fiction?" 41 : 728-730, 1997

2 Marshall, B.J., "Urea protects Helicobacter (Campylobacter) pylori from the bactericidal effect of acid" 99 : 697-702, 1990

3 Rossi, G., "Therapeutic vaccination against Helicobacter pylori in the beagle dog experimental model: safety, immunogenicity, and efficacy" 72 : 3252-3259, 2004

4 Kabir, S., "The current status of Helicobacter pylori vaccines: a review" 12 : 89-102, 2007

5 Ferrero, R.L., "The GroES homolog of Helicobacter pylori confers protective immunity against mucosal infection in mice" 92 : 6499-6503, 1995

6 Ha, N.C., "Supramolecular assembly and acid resistance of Helicobacter pylori urease" 8 : 505-509, 2001

7 Wu, C., "Protection against Helicobacter pylori infection in mongolian gerbil by intragastric or intramuscular administration of H. pylori multicomponent vaccine" 13 : 191-199, 2008

8 Marchetti, M., "Protection against Helicobacter pylori infection in mice by intragastric vaccination with H. pylori antigens is achieved using a non-toxic mutant of E. coli heatlabile enterotoxin (LT) as adjuvant" 16 : 33-37, 1998

9 Smythies, L.E., "Poliovirus replicons encoding the B subunit of Helicobacter pylori urease protect mice against H. pylori infection" 23 : 901-909, 2005

10 Kusters, J.G., "Pathogenesis of Helicobacter pylori infection" 19 : 449-490, 2006

11 Lee, C.K., "Oral immunization with recombinant Helicobacter pylori urease induces secretory IgA antibodies and protects mice from challenge with Helicobacter felis" 172 : 161-172, 1995

12 Nystrom, J., "Oral immunization with HpaA affords therapeutic protective immunity against H. pylori that is reflected by specific mucosal immune responses" 25 : 2591-2598, 2007

13 Ermak, T.H., "Immunization of mice with urease vaccine affords protection against Helicobacter pylori infection in the absence of antibodies and is mediated by MHC class II-restricted responses" 188 : 2277-2288, 1998

14 Carlsohn, E., "HpaA is essential for Helicobacter pylori colonization in mice" 74 : 920-926, 2006

15 Eaton, K.A., "Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets" 59 : 2470-2475, 1991

16 Sutton, P., "Effectiveness of vaccination with recombinant HpaA from Helicobacter pylori is influenced by host genetic background" 50 : 213-219, 2007

17 Thoreson, A.C., "Development of a PCR-based technique for detection of Helicobacter pylori" 10 : 325-333, 1995

18 Correa, P., "Carcinogenesis of Helicobacter pylori" 133 : 659-672, 2007

19 Li, M.F., "A candidate oral vaccine to Helicobacter pylori fusion protein of HspA and CtxB" 33 : 360-364, 2001