Attachment and Biofilm Formation of Pseudomonas fluorescens PSD4 Isolated from a Dairy Processing Line

다국어 초록 (Multilingual Abstract)

The effects of different nutrient sources andtemperatures on attachment and biofilm formation ofPseudomonas fluorescens PSD4, a dairy isolate, werestudied. Initial adherence and attachment capabilitiesamong different strains were studied using microtitre plateassays. Biofilm development was observed using confocalmicroscopy. Strongly adherent cells were seen in proteinrich media. Citrate as a carbon source enhanced biofilmformation. Glucose did not favor biofilm development.
Psychrotrophic P. fluorescens PSD4 formed stronglyadherent biofilms having high metabolic activities at lowtemperatures. P. fluorescens PSD4 with spoilage potentialwas capable of forming strong biofilms in dairy processingenvironments. Biofilm formation was influenced bynutrient availability and growth conditions. These factorsshould be considered for design of effective anti-biofilmstrategies.

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