加減茵蔯蒿湯 水鍼液이 膽道結紮로 유도된 肝損傷에 미치는 영향 = The Effects of the Water Extract of Gagaminjinhotang on the liver Damage Induced by Bile Duct Ligation

국문 초록 (Abstract)

연구배경 : 肝癌 및 肝硬變은 韓國人의 疾病으로 인한 중요한 喪亡原因의 하나이며, 특히 우리나라 B型肝炎의 流行地域으로 急慢性 肝炎이나 肝經辨證, 肝癌의 發病率이 높다. 그리하여 肝硬變症을 寢具治療法중 水鍼을 이용하여 肝의 募穴인 期門穴에 投與하면 治療效果가 있는지에 대하여, 그리고 藥量에 따라 治療效果가 달라지는 지에 대하여 알아보기 위하여 본 연구를 시행하였다.

방법 : 加減茵蔯蒿湯 水鍼液을 膽道를 結紮한 白鼠에 26guage의 1ml 주사기를 이용하여 투여하였다. 經穴은 人體의 期門에 相應하는 左石部位와 腹部內의 任意의 部位를 設定하여 매일 1회 7주간 수침하였다.

결과 : 體重과 Total Bilirubin은 實驗群 A, C에서 有意性 있는 增加와 減少를 보였고, WBC·ALT·AST·ALT 및 Cholesterole은 全實驗群에서 有意性 있는 減少를, Albumin은 全實驗群에서 有意性 있는 增加를 각각 나타내었으며, 組織學的 觀察도 全實驗群에서 有意性을 나타내었다. 또한 Hydroxyproline도 實驗群 A, B에서 有意性있는 減少를 보였다.

결론 : 加減茵蔯蒿湯 水鍼液을 膽道를 結紮한 白鼠의 期門穴에 投與한 缺課 有意한 효과를 보였으나 水鍼量에 따라 有意性의 차이가 있어 敵意한 水鍼量을 투여하는 것이 治療시 重要하다고 思料되며, 또한 水鍼에 대한 安全性 問題에 대하여서도 더욱 硏究할 필요가 있다.

다국어 초록 (Multilingual Abstract)

In order to study the effects of the water extract of Gagaminjinhotang in the Meridian points Liv 14 on the liver damage indued by bile duct ligation during 7 weeks after the operation of bile duct ligation, blood was taken from the heart and body weight, WBC, RBC, hemoglobin, hematocrit, total bilirubin, ALT, AST, ALP, cholesterol, albumin and hydroxyproline were in each experimental apparatus.
The results of this study were as follows;
1. Body weight of EXA, C were significantly increased by the water extract of Gagaminjinhotang.
2. WBC of EXA,B,C were significantly decreased by the water extract of Gagaminjinhotang.
3. RBC, hemoglobin and hematocrit of EXA,B,C were not significantly influenced by the water extract of Gagaminjinhotang.
4. Total bilirubin of EXA,C and direct bilirubin of EXA,B,C were significantly decreased by the water extract of Gagaminjinhotang.
5. ALT, AST,ALP of EXA,B,C were significantly decreased by the water extract of Gagaminjinhotang.
6. Cholesterol of EXA,B,C, were significantly decreased by the water extract of Gagaminjinhotang.
7. Albumin of EXA,B,C, were significantly increased by the water extract Gagaminjinhotang.
8. Hydroxyproline of EXA,B were significantly decreased by the water extract Gagaminjinhtang.
9. On the histological observation, the results of the experimental groups showed significant hepatoprotective effects compared with control group from 3 weeks after the administration of the water extract of Gagaminjinhotang.

According to the above result, it is suggested that the water extract Gagaminjinhotang was significantly effective in WBC, bilirubin, ALT, AST, ALP, cholesterol, albumin and hydroxyproline level and then it seems to be applicable to the fibrosis or cirrhosis of liver.