2LA-8 Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells = 2LA-8 Development of a novel microbubble-liposome complex conjugated with peptide ligands targeting IL4R on brain tumor cells

다국어 초록 (Multilingual Abstract)

SF6-filled microbubbles were prepared by emulsion and solventevaporation method. The prepared MBs were further conjugated with doxorubicin-loaded nano-sized liposome and peptide ligands to interleukin-4 receptor for targeting brain tumor cells. The final MB-Lipo (Dox)-IL4RTP had a spherical structure with the mean size of 1,500 nm. The MB-Lipo (Dox)IL4RTP exhibited cellular uptake in U87MG brain tumor cells with frequency ultrasound energy suggesting that MB-Lipo (Dox)IL4RTP provided effective targeting ability for brain tumor cells. In addition, WST-1 assay results showed that MB-Lipo (Dox)IL4RTP inhibited the proliferation of U87MG cells IL4Rdependently. This was confirmed by western blotting of γH2AX, phospho (Ser15)-p53, p53 and p21 which are signal transduction proteins involved in DNA damage response and cell cycle arrest.