기니픽에서 HM10411 의 항원성 = Antigenicity of HM10411 in Guinea Pigs

다국어 초록 (Multilingual Abstract)

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다국어 초록 (Multilingual Abstract)

HM10411 is a recombinant granulocyte-colony stimulating factor (rG-CSF) that has been developing as a drug for neutropenia. In this study, antigenic potential of HM 10411 was examined by active systemic anaphylaxis (ASA) in guinea pigs and passive cutaneous anaphylaxis (PCA) in guinea pig-guinea pig system. HM10411 was subcutaneously administered at 0, 5, and 50 ㎎/㎏ and also as a suspension with adjuvant (50 ㎎/㎏+FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control responses. In the ASA test, no symptoms except urination and evacuation that were considered as physiological phenomena were observed at 0 and 5 ㎎/㎏. Two of 5 animals at 50 ㎎/㎏ showed sneezing, dyspnea, or cyanosis. All animals in the adjuvant mixture group showed severe symptoms of anaphylatic shock and 3 of them died. In the PCA test, no antibody against HM10411 was detected in the sera from the animals sensitized with 0 or 5 ㎎/㎏. Only 1 serum sample from the animals immunized with 50 ㎎/㎏ showed positive reaction against HM10411, while all 5 sera collected from the HM10411 and FCA mixture group contained the HM10411 -specific antibodies. These results suggest HM10411 is considered to have antigenicity in guinea pig.