Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Oocytes Improves Subsequent Parthenogenetic Development = Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Porcine Oocytes Improves Subsequent Parthenogenetic Development

다국어 초록 (Multilingual Abstract)

Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10 μg/ml) for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to 6.25 μM calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to 10 μg/ml cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to 6.25 μM calcium ionophore for 2 min with 10 μg/ml cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.

참고문헌 (Reference)

1 "Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes. Biol Reprod 40" 1257-1263, 1989

2 "The induction of reversible and irreversible chromosome decondensation by protein synthesis inhibition of meiotic maturation of mouse oocytes. Dev Biol 97" 291-301, 1983

3 "Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine" 86 : 297-304, 2005

4 "Parthenogenetic development of bovine oocytes matured in vitro for 24h and activated by ethanol and cycloheximide. Mol Reprod 38" 380-385, 1994b

5 "Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis. J Embryol Exp Morphol 43" 157-166, 1978

6 "Parthenogenesis of rabbit oocytes activated by different stimuli." 70 : 267-276, 2002

7 "Nuclear dynamics of parthenogenetic development of bovine oocytes matured in vitro for 20 and 40h and activated by combined by ethanol and cycloheximide treatment. Mol Reprod 37" 61-68, 1994a

8 "Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage." 63 : 1277-1289, 2005

9 "Iwamura S Birth of piglets derived from porcine zygotes cultured in a chemically defined medium." 66 : 112-119, 2002

10 "In vitro development of preimplantation porcine nuclear transfer embryos cultured in different media and gas atmospheres." 61 : 1125-1135, 2003

11 "Improvement of an electrical activation protocol for porcine oocytes." 66 : 635-641, 2002

12 "Improved activation by combined cycloheximide and electrical pulse treatment of bovine follicular oocytes matured in vitro for 23-24h. Biol Reprod" 1992

13 "Effects of duration, concentration, and timing of ionomycin and 6-dimethylaminopurine (6-DMAP) treatment on activation of goat oocytes" 71 : 380-388, 2005

14 "Effect of culture conditions on artificial activation of porcine oocytes matured in vitro." 8 : 1153-1156, 1996

15 "Dynamic changes in meiotic progression and improvement of developmental competence of pig oocytes in vitro by follicle stimulating hormone and cycloheximide." 72 : 406-, 2005

16 "Development of cloned pig embryos by nuclear transfer following different activation treatments." 70 : 308-313, 2005

17 "Culture of pig embryos. J Reprod Fertil 48" 61-73, 1993

18 "Comparison of microinjection (piezo-electric) and cell fusion for nuclear transfer success with different cell types in cattle" 4 : 189-196, 2002

19 "Cloned pigs produced by nuclear transfer from adult somatic cells." 407 : 505-509, 2000

20 "Age dependence of bovine oocytes activation. Gamete Res 22" 265-275, 1989

21 "Activation of pig oocytes using calcium ionophore effect of protein synthesis inhibitor cycloheximide." 63 : 101-111, 2000

22 "Activation of cumulus-free mouse oocytes. Mol Repred Dev 26" 159-162, 1990

23 "Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis." 253 : 413-421, 1995