악하선에서 분리된 NGF에 의해 유도되는 비만세포 활성화에 미치는 상백피의 억제효과 = INHIBITOR EFFECTS OF CORTEX MORI ON SUBMANDIBULAR GAND-DRIVER-NERVE GROWTH FACTOR-INDUCED MAST CELL ACTIVATION

다국어 초록 (Multilingual Abstract)

Cortex Mori(Norus alba L), the root bark of mulberry tree has been used as an antiphlogistic, diuretic, and expectorant in herbal medicine. Previous studies have demonstrated that Cortex Mori had inhibitory effects on the compound 48/80-induced degranulation and histamine release from rat peritoneal mast cells(RPMC). The purpose of this study is to determine whether Cortex Mori(CM) could inhibit the nerve growth factor(NGF)-induced activation of RPMC in vivo and in vitro. In vitro, various concentrations of NGF(0.1, 1, 10 and 100㎍/ml) were added into the RPMC suspension(??cells/ml) pretreated with lyso-PS at 37℃ for 10minutes. Cortex Mori pretreatment was performed before or after lyso-PS treatment at 37℃ for 10minutes. Histamine in the supernatants and calcium uptake of RPMC were measured by radioisotope enzymatic histamine assay and radioisotope calcium uptake method. In vivo experiment, to estimate the effect of Cortex Mori on NGF-induced cutaneous reaction, various doses of NGF with or without Co
rtex Mori on NGF-induced cutaneous reaction, various doses of NGF with or without Cortex Mori were injected i.d. int the shaved flank of Sprague-Dawley rats, and the blue cutaneous staining induced by Evans’blue injection at the NGF alone Cortex Mori plus NGF injection sites were observed. Results were 1) the NGF-induced degranulation and histamine release of RPMC pretreated with Cortex Mori were significantly inhibited, compared to those of control without Cortex Mori pretreatment,2) Cortex Mori inhibited the NGF-induced cutaneous reaction remarkably. From the above results, it is suggested that Cortex Mori contains some substances with an activity to inhibit the NGF-induced activation of mast cell.