Effect of Topical Application of Intercellular Lipids on Sodium Lauryl Sulphate-Damaged Skin Barrier Function in Dogs

다국어 초록 (Multilingual Abstract)

Ceramide, cholesterol and free fatty acids are the major intercellular lipids, maintaining the integrity of the
skin barrier. However, the roles of these lipids in canine skin barrier function are little known. The aim of this study
was to evaluate the repairing effects of 2% ceramide (CER), 2% cholesterol (CHO), 2% linoleic acid (LIN) and 2%
intercellular lipid mixture (ILM) on damaged canine skin barrier by 1.25% sodium lauryl sulphate (SLS). Transepidermal
water loss (TEWL), skin hydration, skin pH and skin thickness were assessed. Histological profiles and transmission
electron microscopic (TEM) profiles were assessed on day 12. SLS effectively induced the canine skin barrier damage.
TEWL was significantly decreased by topical application of CER and ILM in SLS and vehicle-treated skin on day
8 and 12, respectively (p < 0.05, p < 0.01). By end of the experiment all lipids significantly decreased the TEWL as
compared with SLS and vehicle control, but CER and ILM more significantly decreased the TEWL than LIN and
CHO, respectively (p < 0.01). Skin hydration was significantly increased by CER and ILM during experimental periods
(p < 0.01). Skin pH was significantly decreased by CER, LIN and ILM. In histological profiles, the thickness of the
stratum corneum (SC) was significantly increased by the SC lipids as compared with vehicle and SLS (p < 0.01).
Especially, CER and ILM showed more prominent improvement of barrier recovery. In TEM of the SC, SLS induced
exfoliations of corneodesmosomes in the SC, and CER and ILM effectively protected exfoliations of corneodesmosomes
on SLS-damaged canine skin. These results indicated that topical application of CER and ILM dramatically improved
damaged-skin barrier function by SLS. Also, it was considered that the use of CER or ILM was recommended for
the management of skin barrier dysfunction by irritant and inflammatory skin disorders such as atopic dermatitis.

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