<P> We investigated the role of Ca<SUP>2</SUP> and protein kinases/phosphatases in the stimulatory effect of insulin on glucose transport. In isolated rat adipocytes, the simple omission of CaCl<SUB>2</SUB> from th...
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https://www.riss.kr/link?id=A105379512
1999
-
Insulin ; Ca<SUP>2⁢ ; </SUP> ; PP-1 ; 2-DG uptake ; Adipocyte ; 5 ; 5 -dimethyl BAPTA/AM
518
SCIE,SCOPUS,KCI등재
학술저널
357-364(8쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P> We investigated the role of Ca<SUP>2</SUP> and protein kinases/phosphatases in the stimulatory effect of insulin on glucose transport. In isolated rat adipocytes, the simple omission of CaCl<SUB>2</SUB> from th...
<P> We investigated the role of Ca<SUP>2</SUP> and protein kinases/phosphatases in the stimulatory effect of insulin on glucose transport. In isolated rat adipocytes, the simple omission of CaCl<SUB>2</SUB> from the incubation medium significantly reduced, but did not abolish, insulin-stimulated 2-deoxy glucose (2-DG) uptake. Pre-loading adipocytes with intracellular Ca<SUP>2</SUP> chelator, 5,5 -dimethyl bis (<I>o</I>-aminophenoxy)ethane-N,N,N N tetraacetic acetoxymethyl ester (5,5 -dimethyl BAPTA/AM) completely blocked the stimulation. Insulin raised intracellular Ca<SUP>2</SUP> concentration ([Ca<SUP>2</SUP>]<I><SUB>i</SUB></I>) about 1.7 times the basal level of 72⁑5 nM, and 5,5 -dimethyl BAPTA/AM kept it constant at the basal level. This correlation between insulin-induced increases in 2-DG uptake and [Ca<SUP>2</SUP>]<I><SUB>i</SUB></I> indicates that the elevation of [Ca<SUP>2</SUP>]<I><SUB>i</SUB></I> may be prerequisite for the stimulation of glucose transport. Studies with inhibitors (ML-9, KN-62, cyclosporin A) of Ca<SUP>2</SUP>-calmodulin dependent protein kinases/phosphatases also indicate an involvement of intracellular Ca<SUP>2</SUP>. Additional studies with okadaic acid and calyculin A, protein phosphatase-1 (PP-1) and 2A (PP-2A) inhibitors, indicate an involvement of PP-1 in insulin action on 2-DG uptake. These results indicate an involvement of Ca<SUP>2</SUP>-dependent signaling pathway in insulin action on glucose transport.
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