Gutte-percha 또는 Resilon을 사용한 근관충전이 상아세관 내의 Enterococcus faecalis에 미치는 영향 = THE EFFECT OF CANAL FILLING WITH GUTTA-PERCHA OR RESILON ON ENTEROCOCCUS FAECALIS IN BOVINE DENTINAL TUBULES

국문 초록 (Abstract)

목적은 근관충전이 상아세관 내에 남아있는 세균에 미치는 영향을 알아보고 가터퍼차와 resilon의 밀폐 능력을 상아세관 내에 남아있는 세균의 생존 여부를 통하여 비교하는 것이다.
발치된 우전치로 치아시편에 E. faecalis를 접종하고 제1군은 음성대조군, 제 2군은 양성대조군, 제3군은 ZOE 계통의 근관전색제와 가터퍼차, 제 4군은 레진 계통의 근관전색제와 가터퍼차, 제 5군은 레진 계통의 근관전색제와 resilon으로 근관 충전하였다. 각 시편은 1, 2, 3주 및 4주간 보관한 뒤 round bur로 근관 내를 삭제하여 얻은 상아질을 배양하고 혼탁도를 측정하였다.
1. Nail varnish로 상아세관을 완전히 밀폐한 경우, 상아세관 내의 E. faecalis의 수는 가터퍼차 및 resilon으로 근관 충전 한 경우보다 통계적으로 유의차 있게 감소하였다 (p＜0.05).
2. 레진 계통의 근관전색제와 resilon으로 근관충전 한 군에서는 시간의 흐름에 따라 상아세관 내의 E. faecalis의 수가 유의차 있게 감소하였다(p＜0.05).
3. 가터퍼차와 resilon으로 근관충전한 군 사이에 상아세관 내에 남아 있는 E. faecalis의 생존 정도는 통계학적 유의차가 없었다 (p＞0.05).

다국어 초록 (Multilingual Abstract)

The purpose of this study was to observe the effect of canal filling on the bacteria left in the dentinal tubules and to compare the sealing ability between Gutta-percha and Resilon. The bovine dentin block models were prepared. E. faecalis was inoculated to dentin blocks and incubated. The dentin blocks were divided into 5 groups.
Group 1 was the negative control. Group 2 was the positive control. Group 3 was filled with ZOE based sealer and Gutta-percha, Group 4 with resin based sealer and Gutta-percha, and Group 5 with resin based sealer and Resilon. After 24 hour, the blocks were incubated at 37℃ for 1, 2, 3 and 4 weeks on BHI agar plates.
The internal dentin portion of the blocks was removed using ISO 027, 029, 031, 035 round burs and the dentin chips were incubated at 37℃ for 24 hour. Following incubation, the optical density of the medium was measured. The data were statistically analysed using repeated measures ANOVA and one-way ANOVA and one-way ANOVA.
The results were as follows,
1. There was statistically significant reduction in the number of E. faecalis of the group where dentinal tubules were completely sealed with nail varnish in comparison with the groups obturated with gutta-percha or resilon (p ＜ 0.05).
2. In group 5, the number of E. faecalis in the dentinal tubules decreased significantly with time (p＜0.05), whereas in Group 3 and 4, there was no reduction in its number (p＞0.05).
3. Under the conditions of this experiment, E. faecalis survived up to 4 weeks after obturation with gutta-percha or resilon (p＞0.05).

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