Development of Indicators to Assess the Stability of Remnant Blood Samples Stored in a Biobank: Experience at One Institution

다국어 초록 (Multilingual Abstract)

Background : One of the major concerns with biobanking is the absence of standard operating procedures to eliminate pre-analytical variation arising from sample collection, preparation, and storage. Currently, there is a lack of tools to carry out quality control procedures for stored blood samples. The aim of this study is to assess the quality of stored blood samples in our biobank and to suggest appropriate indicators for their quality control.
Methods : The stored blood samples that we tested have been registered into our biobank since 2003. These were transferred to our biobank after carrying out routine requested tests, because the samples would have otherwise been discarded. For the purpose of quality control, we analyzed the concentrations and the integrity of DNA and RNA extracted from the stored samples and tested the levels of several serum proteins; the results were compared with the corresponding pre-storage levels.
Results : A total of 19 samples were stored from 2006 to 2009. Of the 22 samples stored between 2003 and 2005, 50% showed complete DNA integrity. However, sufficient RNA integrity was noted in only 1 sample stored as recently as 2009. High blood urea nitrogen levels were also noted in the stored sera, but the increase did not correlate to the duration of storage.
Conclusions : The amount and integrity of nucleic acids extracted from stored blood samples are potential indicators that can be used for quality control. A guideline for the quality assessment of stored blood samples in a biobank is urgently needed. (Korean J Lab Med 2010;30:718-25)

참고문헌 (Reference)

1 대한진단검사의학회, "진단검사의학" 이퍼블릭 821-824, 2009

2 대한진단검사의학회, "진단검사의학" 이퍼블릭 9-16, 2009

3 김순호, "진단검사의 활용" 서울의학사 135-940, 2006

4 국가지정인체유래검체거점은행, "조직도"

5 동결폐조직은행, "인체유래검체거점은행소개"

6 유수진, "분변 검체의 분석 전 보관 상태가 분변잠혈반응검사 결과에 미치는 영향" 대한진단검사의학회 29 (29): 262-267, 2009

7 Imbeaud S, "Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces" 33 : e56-, 2005

8 Fajardy I, "Time course analysis of RNA stability in human placenta" 10 : 21-, 2009

9 Peakman TC, "The UK Biobank sample handling and storage validation studies" 37 (37): i2-i6, 2008

10 Elliott P, "The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine" 37 : 234-244, 2008

11 Schroeder A, "The RIN: an RNA integrity number for assigning integrity values to RNA measurements" 7 : 3-, 2006

12 Boyanton BL Jr, "Stability studies of twenty-four analytes in human plasma and serum" 48 : 2242-2247, 2002

13 Gislefoss RE, "Stability of selected serum proteins after long-term storage in the Janus Serum Bank" 47 : 596-603, 2009

14 Strand C, "RNA quality in frozen breast cancer samples and the influence on gene expression analysis--a comparison of three evaluation methods using microcapillary electrophoresis traces" 8 : 38-, 2007

15 Ribeiro-Silva A, "RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies" 8 : 118-, 2007

16 Raman T, "Quality control in microarray assessment of gene expression in human airway epithelium" 10 : 493-, 2009

17 Creighton TE, "Protein folding" 270 : 1-16, 1990

18 Voet D, "Post-translational modification in Biochemistry" John Wiley & Sons, Inc. 1009-, 1995

19 Friede A, "National biospecimen network blueprint" Constella Group, Inc. 29-48, 2003

20 National Cancer Institute, "National Institute of Health, U.S. Department of Health and Human Services. National Cancer Institute best practices for biospecimen resources"

21 Meyer S, "Messenger RNA turnover in eukaryotes:pathways and enzymes" 39 : 197-216, 2004

22 "International Society for Biological and Environmental Repositories" ISBER

23 Tate J, "Interferences in immunoassay" 25 : 105-120, 2004

24 Zhang DJ, "Effect of serum-clot contact time on clinical chemistry laboratory results" 44 : 1325-1333, 1998

25 Scherczinger CA, "DNA extraction from liquid blood using QIAamp" 42 : 893-896, 1997

26 National Cancer Institute, "Cancer human biobank"

27 Holland NT, "Biological sample collection and processing for molecular epidemiological studies" 543 : 217-234, 2003

28 International Society for Biological and Environmental Repositories, "2008 best practices for repositories: collection, storage, retrieval and distribution biological materials for research. 2nd ed" International Society for Biological and Environmental Repositories 23-27, 2007