Application of High-speed Counter-current Chromatography for the Isolation of 5 Alkaloids from Lotus (Nelumbo nucifera Gaertn.) Leaves

다국어 초록 (Multilingual Abstract)

A high-speed counter-current chromatography (HSCCC) method was developed for isolation of 5alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves.
The 2-phase solvent system of HSCCC composed of light petroleum-ethyl acetate-methanol-water was set up in 2-step separation process in the proportion of 3:5:3:5 for isolation of N-nornuciferine and armepavine, and in the proportion of 1:5:1:5 for that of anonaine, pronuciferine,and nuciferine. The purity of anonaine (14.6mg), pronuciferine (29.7 mg), N-nornuciferine (31.4 mg), nuciferine (22.1 mg),and armepavine (23.3 mg) isolated from 150 mg crude extract of lotus leaves were examined as 95.6, 88.2, 92.5,94.3, and 92.1%, respectively.

참고문헌 (Reference)

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