Endogenous hormone levels and activities of IAA-modifying enzymes during adventitious rooting of tree peony cuttings and grafted scions

다국어 초록 (Multilingual Abstract)

During rooting of both cuttings and grafted scions of tree peony ( Paeonia suff ruticosa Andr.) cultivar ‘Fengdanbai’, the anatomicalstructure, the contents of endogenous hormones (indole acetic acid, IAA; zeatin, ZT; gibberellin, GA 3 ; and abscisicacid, ABA), and the activities of IAA oxidase (IAAO), peroxidase (POD), and polyphenol oxidase (PPO) were observed. Theresults showed that the rooting processes in cuttings and grafted scions were similar, but that the critical period of rootingin cuttings was earlier than in grafts (cuttings: 18–20 days; scions: 38–40 days). IAA content showed a direct correlationwith rooting, with the peak value appearing as the adventitious roots were breaking through the epidermis in both cuttingsand grafts (cuttings: 20 days; scions: 38 days). ZT and GA 3 promoted adventitious rooting, and ABA inhibited it. The peakvalues of the IAA:ABA and IAA:ZT ratios appeared at the same time as the IAA peak (cuttings: 20 days; scions: 38 days),which indicated that these values were closely related to the occurrence of adventitious roots. Changes in the activitiesof IAAO, POD, and PPO, which modify IAA levels, indicated that there were no direct relationships between these threeenzymes and adventitious root formation in cuttings or grafts. These results provide a basis for further study of adventitiousroot formation in the ornamental tree peony.

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