BACKGROUND: Legg-Calve´-Perthes disease (LCPD) is still a refractory disease in children’s orthopedics. With the introduction of the concept of ‘‘osteoimmunology’’, the immune-inflammatory mechanisms between bone and immune system have beco...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A108934522
Yu Ronghui (Department of Orthopedics, First Affiliated Hospital of Nanchang University) ; Ma Cong (Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ; Li Guoyong (Department of Orthopedics, First Affiliated Hospital of Nanchang University) ; Xu Jianyun (Department of Orthopedics, First Affiliated Hospital of Nanchang University) ; Feng Dan (Department of General Surgery, Jiangxi Provincial Children’s Hospital) ; Lan Xia (Department of Orthopedics, First Affiliated Hospital of Nanchang University)
2023
English
KCI등재,SCIE,SCOPUS
학술저널
489-501(13쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
BACKGROUND: Legg-Calve´-Perthes disease (LCPD) is still a refractory disease in children’s orthopedics. With the introduction of the concept of ‘‘osteoimmunology’’, the immune-inflammatory mechanisms between bone and immune system have beco...
BACKGROUND: Legg-Calve´-Perthes disease (LCPD) is still a refractory disease in children’s orthopedics. With the introduction of the concept of ‘‘osteoimmunology’’, the immune-inflammatory mechanisms between bone and immune system have become a research focus of LCPD. However, few studies have reported on the pathological role of inflammation- related receptors such as toll-like receptors (TLRs) as well as immune cells such as macrophages in LCPD. This study was for investigating the mechanism of TLR4 signaling pathway on the direction of macrophage polarization and the repair of avascular necrosis of femoral epiphysis in LCPD.
METHODS: With GSE57614 and GSE74089, differentially expressed genes were screened. Through enrichment analysis and protein–protein interaction network, the functions of TLR4 were explored. Furthermore, immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), hematoxylin & eosin (H&E) staining, micro-CT, tartrate-resistant acid phosphatase (TRAP) dyeing and western blotting were performed for determining the influences of TAK-242 (a TLR4 inhibitor) on the repair of avascular necrosis of femoral epiphysis in rat models.
RESULTS: Totally 40 co-expression genes were screened as well as enriched in TLR4 signaling pathway. Immunohistochemistry and ELISA analyses certified that TLR4 facilitated macrophage polarization toward the M1 phenotype and prevented macrophage polarization toward the M2 phenotype. Besides, the results of H&E and TRAP staining, micro-CT, and western blotting showed that TAK-242 can inhibit osteoclastogenesis and promote osteogenesis.
CONCLUSION: Inhibition of TLR4 signaling pathway accelerated the repair of avascular necrosis of femoral epiphysis by regulating macrophage polarization in LCPD.
Enhancement of Immune Responses Elicited by Nanovaccines through a Cross-Presentation Pathway
Relationship between Tissue Regeneration and Immune Modulation
Nanoparticle-Based Chimeric Antigen Receptor Therapy for Cancer Immunotherapy
Intestinal Peyer’s Patches: Structure, Function, and In Vitro Modeling