옥수수 유래 아라비노자일란 함유 다당체가 자연면역 활성에 미치는 영향

국문 초록 (Abstract)

본 연구에서는 찰옥수수 가공 부산물인 옥수수침지액(CSL)에서 추출한 수용성 다당체(CBP-1S)가 대식세포 및 수지상세포에서 면역 활성을 유도함을 밝혔다. CBP-1S를 처리한 대식세포에서는 NO 생성이 유도되었으며, NO 합성효소인 iNOS와 자연면역 활성에 중요한 COX-2와 다양한 면역활성 사이토카인의 발현이 유도되었다. 또한 CBP-1S를 경구 투여한 마우스의 비장에서는 IL-12를 발현하는 수지상세포의 비율이 증가하였으며, 이는 종양의 증식과 전이를 억제하는 NK 및 T 세포 활성에서 중요한 역할을 수행할 것이라 기대된다. CBP-1S의 주성분인 아라비노자일란은 TLR4 수용체를 통한 신호전달을 매개하는 것으로 생각되며, 이 물질이 MAPK의 인산화를 유도하고 NF-κB를 통한 전사활성에 촉진하는 것을 확인하였다. 이러한 결과들은 아라비노자일란을 포함하는 옥수수유래 다당체를 면역기능 향상을 위한 기능성 식품 원료 등으로 활용할 가능성을 제시한다.

다국어 초록 (Multilingual Abstract)

A water-soluble polysaccharide fraction derived from corn steep liquor, called corn byproduct polysaccharide-1-supernatant (CBP-1S), was analyzed for its immune activity in innate immune cells. Murine peritoneal macrophages, Raw264.7 cells, were treated with CBP-1S, which induced nitric oxide production in a dose dependent manner. The CBP-1S treatment also increased the inducible isoform of nitric oxide synthase and cyclooxygenase-2 as well as cytokine gene expression involved in the innate immune response. In addition, the proportion of dendritic cells expressing interleukin-12 was higher in the splenocytes of mice administered CBP-1S orally, which is expected to play an important role in the natural killer and T cell activity that inhibits tumor proliferation and metastasis. Arabinoxylan, a major component of CBP-1S, induced the phosphorylation of mitogen-activated protein kinase and promoted transcriptional activity through nuclear factor-kB. Therefore, this compound is believed to mediate signal transduction through the toll-like receptor 4 receptor. These results suggest the possibility of using corn-derived polysaccharides containing arabinoxylan as a functional food ingredient for improving the immune function.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

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