DAMR-PCR 마커를 이용한 피 ( Echinochloa crus-galli) 유전자원 다양성 분석 = Utilization of a Set of High-Polymorphism DAMD-PCR Markers for Biodiversity Analysis of Echinochloa crus-galli Germplasm Collection

다국어 초록 (Multilingual Abstract)

This study evaluated the effectiveness of directed amplification of minisatellite-region DNA (DAMD) markers in assessing polymorphism and relationships of 69 Echinochloa crus-gali accessions that preserved in the National Agrobiodiversity Center. Using these primers, a total of 113 bands were amplified, of which 65 polymorphic bands were discovered and the percentage of polymorphic bands (PPB) was 57.52%. SPAR profile data were scored for the computation of pairwise distances as well as a Neighbor Joining (NJ) tree of all the genotypes. From the profiles obtained with all the 12 primers considered together, 65 polymorphism bands were scored. The averaged values of gene diversity (HE) and polymorphism information content (PIC) for each DAMD marker were 0.213 and 0.179 within populations, respectively. The results indicate the low levels of the genetic diversity present amongst the genotypes. The 69 Echinochloa crus-galli germplasm accessions were divided into four different groups, and data analysis identified phenetic groups that were in agreement with those obtained according to agronomic traits and DAMD markers. The present results provide evidence of divergence between cultured and wild type barnyard grass. The cultured barnyard grass was seven accessions that had been cultivated in Korea and have been cultivating in India among the 69 Echinochloa crus-gali accessions. The selected seven accessions have thicker culm diameter and bigger seed size than the other groups, and is one of the putative germplasm resources to develop the new forage crop and phytoremediation.