햄스터 난소세포에서 Daidzein과 Genistein에 의해 유도된 산화적 스트레스에 대한 Vitamin C의 효과

김민혜,김안근,Kim, Min-Hye,Kim, An-Keun 대한약학회 2007 약학회지 Vol.51 No.4

The oxidative stress causes many diseases like cancer, aging, cardiovascular disease, degenerative neurological disorders (Parkinson’s disease, and Alzheimer's disease) by damage of cell membrane, protein deformation, and damage of DNA due to the oxidation of lipid of cell membrane, protein of tissue or enzyme, carbohydrate, and DNA. It is caused by the reactive oxygen species (ROS) that is produced in the metabolic process of oxygen in cell. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cell systemize the antioxidative enzymes to control the oxidative stress. In this research, it is measured that the survival rate of cell by the typical isoflavonoid of daidzein or genistein, activity of antioxidative enzyme, and ROS level, in order to study the effect of isoflavonoid over the ROS production in cell and antioxidative system. As the similar action of the isoflavonoid with the estrogen is examined, women are encouraged to get bean. In view of this trend, it is very important to find out a combination medicine that lowers the oxidative stress caused by the daidzein in the ovarian cell. In the combined treatment of the typical antioxidant of vitamin C to oxidative stress which induced by daidzein recover the control level particularly lowering the ROS in cell by 30%. However, it made no effect in the combined treatment with genistein. Therefore, the research took the combination effect of daidzein with vitamin C in order to check it effect over the antioxidative system. In conclusion, it was disclosed that the oxidative stress caused by daidzein is related to the lowering activity of SOD, and the specific combination effect of daidzein with vitamin C is related to the recovery of SOD activity.

Genistein이 햄스터 난소세포의 항산화효소활성과 발현에 미치는 영향

김민혜,김안근,Kim, Min-Hye,Kim, An-Keun 대한약학회 2007 약학회지 Vol.51 No.1

Reactive oxygen species (ROS) are produced in the metabolic process of oxygen in cells. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cells systemize the antioxidant enzymes to control the oxidative stress. Genistein is one of the isoflavonoids, and its role in controlling cellular oxidative stress is presently the active issue at question. In this study; we analyzed genistein-induced survival rates of the CHO-K1 cells, activities of antioxidant enzymes, ROS levels, and expression levels of antioxidant enzyme genes in order to investigate the effect of genistein on cellular ROS production and antioxidative systems in CHO-K1 cells. As results, the survival rate of cells was decreased as the dose of genistein increases (12.5${\sim}$200 ${\mu}$M). Genistein increased cellular ROS levels, while it reduced total SOD activities and the expression of CuZnSOD. In conclusion, we suggest that genistein may induce oxidative stress via down-regulation of SOD.

성인에서 기관지과민성과 폐기능의 지표로서 High-Sensitivity C-Reactive Protein의 의미

김민혜 ( Min Hye Kim ),김세훈 ( Sae Hoon Kim ),강혜련 ( Hye Ryun Kang ),박흥우 ( Heung Woo Park ),장윤석 ( Yoon Seok Chang ),김동희 ( Dong Hee Kim ),조상헌 ( Sang Heon Cho ),민경업 ( Kyung Up Min ),김유영 ( You Young Kim ),김선신 대한천식알레르기학회 2009 천식 및 알레르기 Vol.29 No.2

Background: Bronchial hyperresponsiveness is a characteristic feature of asthma and closely related with airway inflammation in part. Serum high-sensitivity C-reactive protein (hs-CRP) is a very sensitive marker for systemic inflammation, and several studies has reported the association between hs-CRP and bronchial hyperresponsiveness. Objective: The objectives of this study are to evaluate the association between hs-CRP and bronchial hyperresponsiveness or lung function in healthy Korean subjects. Method: A total of 7,709 subjects underwent methacholine bronchial provocation tests in Seoul National University Hospital health care Gangnam center from October 2003 to January 2009. Among them, 508 subjects were positive for the methacholine bronchial provocationtest. Another 508 subjects negative for the test were enrolled as control, who were matched for age, sex and body mass index. We reviewed the medical records and compared serum hs-CRP levels and the results of the methacholine bronchial provocation test and pulmonary function tests. Result: Bronchial hyperresponsiveness was more frequently observed in subjects with higher hs-CRP levels (group IV). (24.6% vs. 14.8%) than in subjects with lower hs-CRP levels (group I). There was a negative correlation with hs-CRP levels and FEV1, FVC or FEV1/FVC. Conclusion: There was a negative association between serum hs-CRP levels and bronchial hyperresponsiveness or lung function. (Korean J Asthma Allergy Clin Immunol 2009;29:112-116)

인슐린 알레르기 환자에서 탈감작 후 나타난 인슐린 특이항체의 변화

김민혜 ( Min Hye Kim ),임경환 ( Kyung Hwan Lim ),박한기 ( Han Ki Park ),김병근 ( Byung Keun Kim ),강민구 ( Min Koo Kang ),권재우 ( Jae Woo Kwon ),김태완 ( Tae Whan Kim ),정재우 ( Jae Woo Jung ),이소희 ( So Hee Lee ),이상민 ( Sang 대한천식알레르기학회 2010 천식 및 알레르기 Vol.30 No.1

Although human insulin is widely used, insulin allergy is still an important issue. Desensitization is one of the strategies to overcome insulin allergy, but there are few report concerning the mechanism of immune tolerance to insulin achieved by desensitization. Based on the previous studies regarding immunotherapy, it can be understood that changes in allergen specific antibody may be a probable mechanism. We report time-serial changes in insulin-specific antibodies after successful desensitization of type I hypersensitivity to insulin. A 68-year-old man visited our clinic due to dyspnea, loss of consciousness and whole body rash with erythema immediately after the injection of human insulin. The patients showed positive skin reactions to all available human insulins. Enzyme linked immunosorbent assay (ELISA) demonstrated that human insulin specific IgE level increased. We injected human insulin (aspart) to patient subcutaneously with gradual increase manners and finally achieved successful insulin desensitization without serious systemic reactions. Human insulin-specific antibody was measured 4, 8 and 12 weeks after desensitization, which showed interesting time-serial changes; specific IgE gradually decreased, while IgG1, IgG2 and IgG4 gradually increased. (Korean J Asthma Allergy Clin Immunol 2010; 30:63-67)

고밀토지이용 주변 지역의 단독주택지 개발특성과 과제

김민혜(Kim Min-Hye),박종안(Park Jong-An),채병선(Chai Byung-Sun) 한국도시설계학회 2007 도시설계 : 한국도시설계학회지 Vol.8 No.3

디카르복실 산의 염료감응형 태양전지의 효율 향상 효과

김민혜 ( Min Hye Kim ),권영욱 ( Young Uk Kwon ) 한국공업화학회 2006 응용화학 Vol.10 No.1

식품알레르기 및 아토피피부염에서 마이크로바이옴 연구

김민혜 ( Min-hye Kim ),서동인 ( Dong In Suh ),이수영 ( Soo-young Lee ),김윤근 ( Yoon-keun Kim ),조영주 ( Young-joo Cho ),조상헌 ( Sang-heon Cho ) 대한천식알레르기학회(구 대한알레르기학회) 2016 Allergy Asthma & Respiratory Disease Vol.4 No.6

Food allergy (FA) and atopic dermatitis (AD) are representative allergic diseases that begin early in life and result in considerable socioeconomic burden. While the pathophysiology and the optimal treatment modalities of these diseases are largely unknown, the role of microbes in health and disease are being highlighted. Recent advances in analyzing microbiome have enabled us to expand our research on impacts of the microbiome on the onset and course of FA and AD. Risk factors that are presumed to affect intestinal microbiome also modulate the onset of allergic diseases, which is more evident in AD than in FA. Considering animal studies, intestinal microbiota interacts with FA and the influence is bi-directional. The activation of regulatory T cell and the innate immune system is supposed to mediate the interaction. Regarding human studies, there exists the difference in the composition of microbiome between subjects with FA or AD and matched normal controls, which can further play as a predictive marker for later development of FA or AD. Probiotics are now investigated as a primary therapeutic agent or as an adjuvant tool for conventional therapies in preventing or modulating FA or AD. Currently, several reports on favorable outcomes become available, which should be replicated and backed up by large-scale studies with more detailed protocols. (Allergy Asthma Respir Dis 2016:4:389-398)

정신병적 증상을 동반한 메스암페타민 사용자들의 임상적 특성

김민혜(Min-Hye Gim),권성민(Sung-Min Kwon),김양태(Yang-Tae Kim),조성남(Sung-Nam Cho),권도훈(Do-Hoon Kwon) 한국중독정신의학회 2006 중독정신의학 Vol.10 No.1

Objectives:The purpose of this study was to clarify the socio-demographic and clinical characteristics such as use pattern, the history of other substances use and preoccupation behavior in methamphetamine users with psychotic features admitted in Bugok National Hospital. Methods:The subjects were 51 hospitalized male patients who met the DSM-IV criteria for methamphetamine abuse or dependence. Psychotic symptoms in methamphetamine users were evaluated using the Brief Psychiatric Rating Scale (BPRS). Their family members were interviewed in order to complete premorbid sociodemographic profiles, the history of other substances use including smoking and alcohol, and use patterns and preoccupation behavior during methamphetamine use. Results:Compared with their non-psychotic counterparts, methamphetamine users with psychotic features were younger in terms of age at first use, first psychiatric hospitalization, and faced socio-occupational problem firstly. In addition, they were admitted to psychiatric hospitals more frequently. Conclusion:Futher large, welldesigned trial will be needed to treat and manage of domestic methamphetamine users with psychotic features.

정부성과가 시민참여에 미치는 영향에 대한 연구

김민혜(Kim, Min Hye),이승종(Lee, Seung Jong) 서울행정학회 2017 한국사회와 행정연구 Vol.28 No.2

정부성과, 시민참여 그리고 정부신뢰에 관한 논의들은 최근 행정학에서 주요하게 다뤄지고 있는 주제이다. 특히 시민참여의 영향요인인 정부신뢰는 행정학 분야에서 다양하게 연구되고 있는 반면, 정부성과가 시민참여에 미치는 영향에 관한 연구는 희박한 실정이다. 본 연구는 이러한 인식에서 출발하여, 정부성과가 시민참여의 유의미한 영향요인인지 밝히는 것을 목적으로 한다. 분석을 위해 본 연구에서는 “2014 정부역할과 삶의 질에 대한 국민인식조사” 데이터를 활용하여, 참여를 선거참여와 비선거참여로 유형화하였고, 정부신뢰를 고려할때 정부성과가 시민참여에 미치는 효과를 검증하고자 했다. 즉, 정부신뢰를 조절변수로 투입하여, 정부신뢰수준에 따라 정부성과가 시민참여에 미치는 효과의 방향과 크기가 차별적으로 나타나는지 분석하였다. 분석결과, 정부성과는 선거참여에 영향을 미치지 못했지만, 비선거참여에서는 통계적으로 유의미한 영향을 미친 것으로 나타났다. 또한 정부신뢰수준에 따라 정부성과의 시민참여에 미치는 효과는 차별적인 것으로 나타나 정부신뢰변수의 조절효과가 확인되었다. 정부에 대한 높은 신뢰를 가진 시민들은 정부성과가 높을수록 참여가 증가하는 것으로 나타났고, 정부에 대한 신뢰수준이 낮은 시민들은 정부성과가 낮을수록 참여가 증가하는 것으로 확인되었다. 그러나 선거, 비선거 참여에 따른 정부신뢰변수의 조절효과의 방향성은 차별적이지 않은 것으로 나타났다. 따라서 본 연구는 정부성과가 시민참여의 주요한 영향요인이며, 정부신뢰가 정부성과와 시민참여 간 인과관계에서 조절변수의 역할을 할 수 있음을 실증연구로 확인했다는데 그 의미가 있다. Government performance, citizen participation and trust in government are critical issues on recent studies. Though the importance on public administration, the effect of government performance on citizen participation has rarely been studied and tested empirically due to a lack of data. Besides, recent studies have never been conducted to use trust in government as a moderator variable. The purpose of this study is to examine how the effect of government performance on citizen participation differs depending on the degree of trust in government. To solve this research question, we set the independent variable as government performance, dependent variable as citizen participation categorized into 2014 local election turnout and non-electoral participation, and trust in government as a moderator variable. We used 2014 survey data of Koreans" recognition toward government conducted by Korea Gallup and have tested a hypothesis using logistic regression and multiple regression analysis. The results indicate that government performance is a statistically significant factor on non-electoral participation. Then, trust in government is included as a moderator variable on the analysis, we confirm its moderating effect on both kinds of citizen participation, but we cannot find differential effect of trust in government regarding two types of participation.

현대 디지털 전시공간에 나타난 뉴미디어 표현특성에 관한 연구

김민혜(Kim, Min-Hye),김문덕(Kim, Moon-Duck) 한국실내디자인학회 2007 한국실내디자인학회 학술대회논문집 Vol.9 No.3