Quantitative trait loci analysis of morphological traits in Citrus

Mehtap S¸ ahin-C¸ evik,Gloria A. Moore 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.1

The objectives of this study were to understand the genetic basis of morphological variation observed in the genus Citrus and its relatives and to identify genomic regions associated with certain morphological traits using genetic linkage mapping and quantitative trait loci (QTLs) analysis with random amplified polymorphic DNA (RAPD) markers. First, a genetic linkage map was constructed with RAPD markers obtained by screening 98 progeny plants from a {Citrus grandis 9 [C. paradisi 9 Poncirus trifoliata]} 9 {[(C. paradisi 9 P. trifoliata) 9 C. reticulata] 9 [(C. paradisi 9 Poncirus trifoliata) 9 C. sinensis]} intergeneric cross. The map contains 69 RAPD markers distributed into nine linkage groups. Then, 17 different morphological traits, including six tree and two leaf characters of 98 progeny plants and six floral and three fruit characters of about half of the same progeny plants were evaluated for 2 years and statistically analyzed for variation. Statistical analysis of individual traits indicated that trunk diameter and growth, tree height, canopy width, tree vigor and growth, leaf length and width, petal and anther numbers, petal length and width, length of pistil and style, fruit length and diameter, and fruit segment number showed normal or close to normal distribution, suggesting that these traits may be inherited quantitatively. Quantitative data from the morphological traits were analyzed to detect markers and putative QTLs associated with these traits using interval mapping method. QTL analysis revealed 18 putative QTLs of LOD[3.0 associated with 13 of the morphological traits analyzed. The putative QTLs were distributed in several different linkage groups, and QTLs associated with similar traits were mostly mapped to the same LG or similar locations in the linkage group, indicating that the same genomic region is involved in the inheritance of some of the morphological traits.

The Effects of Alendronate and Calcitonin on Cytokines in Postmenopausal Osteoporosis: A 6-Month Randomized and Controlled Study

AliG?r,AzizDenli,Remzi?evik,KemalNas,MehmetKarako?,AysegulJaleSara? 연세대학교의과대학 2003 Yonsei medical journal Vol.44 No.1

The isomorphism between two fundamental groups by Cayley graphs

˙I. Naci Cang¨ul,A. Sinan C¸ evik,¨Ozden Koruo˘glu 장전수학회 2008 Advanced Studies in Contemporary Mathematics Vol.17 No.1

LetG1 and G2 be two nite groups and letCay(G1,S1) and Cay(G2,S2) be the corresponding Cayley graphs of these groups, respectively. By [2] and [8], one can dene the fundamental groupπ1(Γ,v) by using any connected graph Γ with a fixed vertex v. In this paper we give sufficient conditions for any two fundamental groups which are obtained by Cayley graphs Cay(G1,S1) and Cay(G2,S2) to be isomorphic. At the final part of the paper, we present some examples of this result.

Complications of Brucellosis in Different Age Groups: A Study of 283 Cases in Southeastern Anatolia of Turkey

AliG?r,MehmetFarukGeyik,BunyaminDikici,KemalNas,Remzi?evik,JaleSara?,SalihHosoglu 연세대학교의과대학 2003 Yonsei medical journal Vol.44 No.1

On the Laplacian-energy-like invariant

Das, Kinkar Ch.,Gutman, Ivan,Ç,evik, A. Sinan Elsevier 2014 Linear Algebra and its Applications Vol.442 No.-

<P>Let G be a connected graph of order n with Laplacian eigenvalues mu(1) >= mu(2) >= ... mu(n-1) >mu(n) = 0. The Laplacian-energy-like invariant of the graph G is defined as LEL = LEL(G) = Sigma(n-1)(i=1)root mu(i) . Lower and upper bounds for LEL are obtained, in terms of n, number of edges, maximum vertex degree, and number of spanning trees. (C) 2013 Elsevier Inc. All rights reserved.</P>

Spinal Cord Compression by Primary Amyloidoma of the Spine

KemalNas,AdemArslan,AdnanCeviz,AslanBilici,AliG?r,MustafaSerdarKemalo?lu,Remzi?evik,Ayseg?lJaleSara? 연세대학교의과대학 2002 Yonsei medical journal Vol.43 No.5

Identification and Expression Analysis of Genes Induced in Response to Tomato chlorosis virus Infection in Tomato

Mehtap Şahin-Çevik,Emine Doguş Sivri,Bayram Çevik 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.3

Tomato (Solanum lycopersicum) is one of the most widely grown and economically important vegetable crops in the world. Tomato chlorosis virus (ToCV) is one of the recently emerged viruses of tomato distributed worldwide. ToCV-tomato interaction was investigated at the molecular level for determining changes in the expression of tomato genes in response to ToCV infection in this study. A cDNA library enriched with genes induced in response to ToCV infection were constructed and 240 cDNAs were sequenced from this library. The macroarray analysis of 108 cDNAs revealed that the expression of 92 non-redundant tomato genes was induced by 1.5-fold or greater in response to ToCV infection. The majority of ToCV-induced genes identified in this study were associated with a variety of cellular functions including transcription, defense and defense signaling, metabolism, energy, transport facilitation, protein synthesis and fate and cellular biogenesis. Twenty ToCV-induced genes from different functional groups were selected and induction of 19 of these genes in response to ToCV infection was validated by RT-qPCR assay. Finally, the expression of 6 selected genes was analyzed in different stages of ToCV infection from 0 to 45 dpi. While the expression of three of these genes was only induced by ToCV infection, others were induced both by ToCV infection and wounding. The result showed that ToCV induced the basic defense response and activated the defense signaling in tomato plants at different stages of the infection. Functions of these defense related genes and their potential roles in disease development and resistance to ToCV are also discussed.

Tax Structure and Economic Growth: A Panel Data from OECD Countries

Savaş Çevik,오창규 경남대학교 산업경영연구소 2013 지역산업연구 Vol.36 No.1

This study examines the long-run relationship between tax structure and GDP per capita by based on panel data of 26 OECD countries over the period 1980-2010. Hausman specification test to verify the differences between PGM and MG was applied and the residuals of the equations was tested for stationary. The results suggest that income taxes are generally associated with lower economic growth, except for corporate income tax. On the other hand, consumption taxes in all types are associated with higher growth rate. In all specifications, taxes on personal income are negatively associated with GDP per capita, while shifting to consumption taxes positively affects the growth. In the perspectives of revenue-neutral analyses, we reveal the robust evidence on favoring consumption taxes over taxes on personal income (the sum of personal income tax, social security contribution and payroll taxes) with respect to growth.

Potentiometric urea biosensor based on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles

Emre Çevik,Mehmet S¸ enel,Abdülhadi Baykal 한국물리학회 2013 Current Applied Physics Vol.13 No.1

Urease enzyme was covalently attached on the poly(glycidylmethacrylate) (PGMA)-grafted iron oxide nanoparticles on Au electrode for the fabrication of urea biosensor. The telomere of poly(- glycidylmethacrylate) (PGMA) with a trimethoxysilyl terminal group was synthesized by telomerization of glycidylmethacrylate. Iron oxide nanoparticles were coated with telomere of poly(- glycidylmethacrylate) in order to obtain good enzyme immobilization platform. The telomere and nanoparticles were characterized by using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and thermal gravimetric analysis (TGA). The biosensor’s potentiometric response was measured as a function of urea concentration in phosphate buffer solution (10 mM, pH 7.5) and showed a linear range of 0.25e5.0 mM urea. The produced biosensor exhibited a good response time of w8 s and was stable for about two months. The basic features (optimum pH, optimum temperature, interference and storage stability) of the enzyme electrode were determined

Detection of Viruses Infecting Stone Fruits in Western Mediterranean Region of Turkey

Bayram Çevik Nejla Yardimci,Handan Çulal-Klllç 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.1

Field surveys were conducted in 45 stone fruit orchards in seven districts of Isparta Province located in western Mediterranean region of Turkey important for stone fruit production. Leaf samples were collected from 175trees showing virus-like symptoms. These samples were first tested by ELISA for five different RNA viruses including Apple mosaic ilarvirus (ApMV), Prunus necrotic ringspot ilarvirus (PNRSV), Prune dwarf ilarvirus (PDV),Plum pox potyvirus (PPV), Apple chlorotic leafspot trichovirus (ACLSV). While no ApMV and PPV infection was found, 46, 24 and 16 samples were tested positive for PDV, ACLSV and PNRSV, respectively, in ELISA showing about 45% of symptomatic trees in the region were infected with at least one of these viruses. In addition,it was found that nine sweet cherry trees were mixed infected with two or three of these viruses and PDV with an infection rate of 26.3% was the most widespread virus in symptomatic trees in western Mediterranean region. Thirty samples were selected and tested by a multiplex RT-PCR (mRT-PCR) for simultaneous detection of these viruses. While PPV was not detected, more than half of the tested 20 samples were individually or mixed infected with ApMV, ACLSV, PNRSV and PDV. The mRT-PCR results were confirmed by detection of these viruses individually in some of the field samples using RT-PCR with primes specific to each virus. Comparison of ELSA and mRT-PCR results of 30 samples showed that numbers of infected and mixed infected samples as well as infection and mixed infection rates were significantly higher in RT-PCR (20 and 66.7%)than in ELISA (14 and 46.7%). The results confirm that mRT-PCR is more sensitive than ELISA.