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Lee, Jae-Seo,Yoon, Suk-Ja,Kang, Byung-Cheol 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.4
Bifid mandibular canal can be an anatomic variation. This condition can lead to complication when performing mandibular anesthesia or during extraction of lower third molar, placement of implants and surgery in the mandible. Four patients underwent preoperative imaging for extraction of third molars using CBCT (CB Mercuray, Hitachi, Japan). The axial images were processed with CBworks program 2.1 (CyberMed Inc., Seoul, Korea). The branches for supplying the lower third molar were identified mainly on cross-sectional and panoramic images of CBCT. Since the location and configuration of mandibular canal variations are important in any mandibular surgical procedures, we report 4 cases of bifid mandibular canal with panoramic and the CBCT images. (Korean J Oral Maxillofac Radiol 2009; 39 : 209-12)
Influences of Carbachol on Potassium Channels in Sublingual Acinar Cells of Rat
Shin, Myung-Sang,Park, Kyung-Pyo,Kim, Joong-Soo,Lee, Jong-Heun The Official Publication of Korean Academy of Oral 1996 International Journal of Oral Biology Vol.20 No.1
The Saliva is formed by outflow of potassium ion after calcium ion influx into acinar cell which was activated by secretagogue. The channel related with the outflow of potassium ion channel is one of the channels first studied with patch clamp technique in epithelial cell. Contrary to the many studies of submandibular gland, there has been no work about the electrophysiological study of sublingual gland with patch clamp technique. The sublinqual acinar cells were collected from Spraue-Dawley rat by time-scheduled trypsin and collagenase treatment. To record channel activities, micropipettes filled with HEPES-buffered high potassium solution with 2-5 MΩresistance were used. Membrane currents were recorded by cell attached patch clamp method. 10 μM Acetylcholine(ACh) or 10 μM Carbachol(CCh) was directly added in perfusion solution. The channel activities were recoreded and were evaluated with personal computer on pClamp software. The obtained results were as followed. 1. We could attain the isolated single acinar cells of sublingual gland. 2. The conductance of potassium ion channel was about 190pS and voltage dependent. 3. The the other type of channel was not sensitive to pipette voltage and had small conductance. 4. Addition of ACh or CCh increased the open probability of potassium channels without change of conductance.