http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Experimental study on the deformation characteristics of RC beam-column subassemblages
Zixiong Guo,Yong Yang 국제구조공학회 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.21 No.4
Cyclic loading tests were carried out on six half-scale reinforced concrete beam-column subassemblages designed to the current Chinese Seismic Design Code for Buildings. The deformation behavior and restoring force characteristics of the subassemblages were studied. Emphasis was directed on their seismic behavior and deformation components. Based on test data and a simplified analysis model of the global and local deformation, the contribution of the deformation components due to beam flexure, column flexure, joint shear, and slippage of longitudinal reinforcement in the joint to the global deformation of subassemblages at different displacement amplitudes of cyclic loading was investigated.
Experimental study on the deformation characteristics of RC beam-column subassemblages
Guo, Zixiong,Yang, Yong Techno-Press 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.21 No.4
Cyclic loading tests were carried out on six half-scale reinforced concrete beam-column subassemblages designed to the current Chinese Seismic Design Code for Buildings. The deformation behavior and restoring force characteristics of the subassemblages were studied. Emphasis was directed on their seismic behavior and deformation components. Based on test data and a simplified analysis model of the global and local deformation, the contribution of the deformation components due to beam flexure, column flexure, joint shear, and slippage of longitudinal reinforcement in the joint to the global deformation of subassemblages at different displacement amplitudes of cyclic loading was investigated.
Zhou, Zixiong,Kim, Jong Won,Zhao, Jing,Qi, Jing,Choi, Seong Jin,Lim, Chae Woong,Lee, Moo-Yeol,Lee, Kyuhong,Kim, Bumseok Elsevier 2018 Toxicology in vitro Vol.52 No.-
<P><B>Abstract</B></P> <P>Accumulative evidence showed that cigarette smoke (CS) detrimentally affects the pathogenesis of nonalcoholic steatohepatitis (NASH). The purpose of this study was to evaluate the effects of CS extract (CSE) or total particulate matter (TPM) on the <I>in vitro</I> steatohepatitis model using mouse primary hepatocytes treated with palmitic acid (PA) or PA plus LPS. Increased hepatocellular damage was observed in PA-treated hepatocytes with TPM or CSE treatment, but increased triglyceride level was only observed in PA plus LPS-treated hepatocytes with a high concentration of TPM. Also, expression levels of steatohepatitis-related genes such as TNF-α, NOS 2, and SREBP-1c were significantly increased after treatment of TPM. To further demonstrate the role of Kupffer cells (KCs) after CS extracts treatment, trans-well co-culture system of hepatocytes and KCs was utilized. The levels of inflammatory cytokines and the ratios of Bax/Bcl-2 (apoptosis-related genes) were markedly increased in co-cultured hepatocytes after TPM or CSE treatment. Interestingly, KCs activation was augmented in KCs upon treatment with CSE or TPM. Overall, our findings indicate that <I>in vitro</I> treatment with CSE or TPM differentially contributes to the severity of steatohepatitis by modulating steatohepatitis-related lipotoxicity and inflammation, which might be caused by KCs activation with subsequent induction of hepatocytes apoptosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 0.4 mM PA treatment could induce steatohepatitis on primary hepatocytes. </LI> <LI> Increased hepatotoxicity was observed in PA-treated hepatocyte after CS treatment. </LI> <LI> CS treatment enhanced PA-induced inflammation and oxidative stress levels. </LI> <LI> TPM treatment increased PA plus LPS-induced lipotoxicity. </LI> <LI> CS enhanced PA-induced apoptosis of hepatocytes when co-cultured with KCs. </LI> </UL> </P>
Evaluation of different media for ex vivo porcine lung culture model
Yang, Myeon-Sik,Zhou, Zixiong,Khatun, Amina,Nazki, Salik,Jeong, Chang Gi,Kim, Won Il,Lee, Sang Myeong,Kang, Seog-Jin,Lim, Chae Woong,Kim, Bumseok The Korean Society of Veterinary Service 2018 韓國家畜衛生學會誌 Vol.41 No.4
Developing drugs targeting respiratory pathogen is essential to control respiratory diseases. Many experiments have been performed under in vivo situation. However, in vivo experiments have economical and ethical issues. The objective of this study was to determine the possibility of developing an ex vivo lung culture system with possible application for respiratory infection studies. After isolating lungs from naïve pigs, agarose-inflated lung tissues were prepared and sliced manually. These sliced lung tissues were then subsequently placed on 24-well plates. Eight different combinations of media were used to determine the optimum ex vivo lung culture condition. In addition, lung tissues were infected with porcine reproductive and respiratory syndrome (PRRS) virus at a titer of $1{\times}10^4\;TCID_{50}/mL$. Virus growth was confirmed by titration in MARC-145 cells at 2, 4, 6 days post infection (dpi). We found that ex vivo lung culture in physiological environment was not media specific based on histopathology and cytotoxicity. However, under virus-infected condition, thickened alveolar walls in the lung tissues and stable virus titers at 2, 4, 6 dpi were shown in F12K medium suggesting that it was useful for tissue maintenance and virus infection using PRRS virus infected lung tissues. The present study shows the possibility of using porcine ex vivo lung model for respiratory infection studies.
Experimental investigation on the shear capacity of RC dapped end beams and design recommendations
Wang, Quanfeng,Guo, Zixiong,Hoogenboom, Pierre C.J. Techno-Press 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.21 No.2
In this paper, the shear resistance behaviour of reinforced concrete (RC) dapped end beams is investigated by 24 tests until failure load. The main parameters considered are the dapped end height, the type and effective range to provided the stirrups and the bent form of the longitudinal reinforcement. The failure behaviour of dapped end beams is presented and some conclusions are given. Inclined stirrups and longitudinal bent reinforcement have more influence on the shear capacity than vertical stirrups. Additionally, the shear mechanism of dapped end beams is analysed. Relatively simple semi-empirical equations for shear strength have been derived based on the results of 22 dapped end beams. The predicted results are in close agreement with the experimental ones. Finally, some design suggestions for the ultimate shear strength of dapped end beams are presented.
Evaluation of different media for ex vivo porcine lung culture model
Myeon-Sik Yang,Zixiong Zhou,Amina Khatun,Salik Nazki,정창기,김원일,이상명,Seog-Jin Kang,임채웅,김범석 한국동물위생학회 2018 韓國家畜衛生學會誌 Vol.41 No.4
Developing drugs targeting respiratory pathogen is essential to control respiratory diseases. Many experi-ments have been performed under in vivo situation. However, in vivo experiments have economical and ethical issues. The objective of this study was to determine the possibility of developing an ex vivo lung culture system with possible application for respiratory infection studies. After isolating lungs from naïve pigs, agarose-inflated lung tissues were prepared and sliced manually. These sliced lung tissues were then subsequently placed on 24-well plates. Eight different combinations of media were used to determine the optimum ex vivo lung culture condition. In addition, lung tissues were infected with por-cine reproductive and respiratory syndrome (PRRS) virus at a titer of 1×104 TCID50/mL. Virus growth was confirmed by titration in MARC-145 cells at 2, 4, 6 days post infection (dpi). We found that ex vivo lung culture in physiological environment was not media specific based on histopathology and cytotoxicity. However, under virus-infected condition, thickened alveolar walls in the lung tissues and stable virus titers at 2, 4, 6 dpi were shown in F12K medium suggesting that it was useful for tissue maintenance and virus infection using PRRS virus infected lung tissues. The present study shows the possibility of using porcine ex vivo lung model for respiratory infection studies.
Γ-Aminobutyric acid promotes methionine-choline deficient diet-induced nonalcoholic steatohepatitis
Seok Roh, Yoon,Cho, Ara,Zhou, Zixiong,Jeong, Hyuneui,Park, Jeong-Eun,Cha, Youn-Soo,Oh, Suk-Heung,Lim, Chae-Woong,Kim, Bumseok Editorial Department of Journal of Biomedical Rese 2017 JOURNAL OF BIOMEDICAL RESEARCH -ELSEVIER- ENGLISH Vol.31 No.1
<P><B>Abstract</B></P><P>Nonalcoholic steatohepatitis (NASH) is one of the most common liver diseases and a major cause of liver fibrosis worldwide. Γ-Aminobutyric acid (GABA) is one of the most abundant inhibitory neurotransmitters in the central nervous system. Recently, it has been reported that GABAergic signaling pathways are found in various non-neuronal tissues including the immune system and play a functional role. In the present study, we investigated whether administration of GABA has effects on NASH through its immunomodulatory effects. To test this hypothesis, C57BL/6 mice were fed a methionine–choline-deficient (MCD) diet for 8 weeks. After four weeks into MCD feeding, mice were provided with plain water (control) or water containing 2 mg/mL of GABA for the subsequent 4 weeks. Using this MCD diet-induced NASH model, we found that mice receiving GABA showed more severe steatohepatitis and liver fibrosis than control mice. This increased liver damage was confirmed by higher levels of serum alanine transaminase (ALT) and aspartate aminotransferase (AST) compared to the control group. In accordance with increased liver steatohepatitis, NASH-related and inflammatory gene expression (collagen α1, tissue inhibitor of metalloproteinase-1, TNF-α) in the liver was markedly increased in GABA-treated mice. Furthermore, GABA directly enhanced production of inflammatory cytokines including IL-6 and TNF-α in LPS activated RAW macrophage cells and increased TIB–73 hepatocyte death. Such effects were abolished when GABA was treated with bicuculline, a competitive antagonist of GABA receptors. These results suggest that oral administration of GABA may be involved in changes of the liver immune milieu and conferred detrimental effects on NASH progression.</P>
Huang Xi,Shen Yinzhong,Li Qingshan,Li Xiaoyan,Zhan Zixiong,Li Guang,Li Zhenhe 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.7
The nanoindentation behavior of P92 steel with thermomechanical treatment under 3.5 MeV Fe13þ ion irradiation at room temperature, 400 and 700 C was investigated. Pop-in behavior is observed for all the samples with and without irradiation at room temperature, while the temperature dependence of pop-in behavior is only observed in irradiated samples. The average load and penetration depth at the onset of pop-in increase as the irradiation temperature increases, in line with the results of the maximum shear stress. Irradiation induced hardening is exhibited for all irradiated samples, but there is a significant reduction in the hardness of sample irradiated at 700 C in comparison to the samples irradiated at room temperature and 400 C. The ratio of hardness to elastic modulus for all samples decreases with increasing penetration depth except for samples at 700 C. With the increasing of irradiation temperature, the ratio of the irreversible work to the total work gradually decreases. In contrast, it increases for samples without irradiation