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水稻品種 “統一”과 “統一찰”의 遺傳背景 Ⅰ. Polyacrylamide gel 等電點電氣泳動에 의한 種子 esterase isozyme의 band 類型
Sun Zik Park(朴淳直),Hoon Kee Kim(金焄基) 한국육종학회 1990 한국육종학회지 Vol.22 No.3
Polyacrylamide gel isoelectric focusing was used to analyze the grain esterase isozymes of rice varieties, Tongil, Tongilchal and their related parents. Six major bands were revealed in the gel part of high pH and observed different banding patterns. Esterase isozyme banding patterns in grains were separated into three types, namely Est-Ⅰ, Est-Ⅱ and Est-Ⅲ in this experiment. Of the varieties analyzed, Japonica showed Est-Ⅰ and Indica was both Est-Ⅰ and Est-Ⅱ, while Japonica×Indica showed Est-Ⅲ, respectively. Esterase isozyme banding patterns of Tongil, Tongilchal, IR8, IR262, IR833, IR1317 and Peta were identical. It was concluded that the source of esterase isozymes genotype in grains of Tongil and Tongilchal was Indica variety Peta.
벼 雄性細胞 培養에 의한 trisomicsdml 移轉과 培養細胞의 再分化에 미치는 過剩染色體의 影響
Sun Zik Park(朴淳直),Gang Sup Lee(李鋼燮),Hee Jong Koh(高熙宗),Mun Hue Heu(許文會) 한국육종학회 1991 한국육종학회지 Vol.23 No.2
This study was carried out to investigate the transmission frequency of the extra chromosomes in rice trisomic plants through male and female gametes including selfed progeny, and to know the effect of extra chromosomes on the male gametic cell culture of rice. the extra chromosomes were transmitted to next generation at the rate of 0.0% to 9.7% through male gamete and 11.3% to 35.7% through female gamete, although the rate was 27.7% to 40.1% in selfed progeny of each trisomic type. Trisomic plant showed different culturability for callus induction, plant regeneration and green tissue appearence depending on the trisomic types in anther culture. Callus induction rates were ranged from 4.7% to 27.3% and plant regeneration rates 3.0% to 22.0%. In anther culture the highest transmission rate of extra chromosomes was 27.8% in trisomic 4(E), while the extra chromosomes of trisomic 12(A), 8(D) and 7(F) were not transmitted through anther culture in this study. The regenerated plants varied in chromosome numbers.
水稻 遺傳子 分析用 遺傳標識基株 育成 Ⅱ. japonica벼 遺傳檢定親으로서 三染色體植物의 育成
Sun Zik Park(朴淳直),Mun Hue Heu(許文會),Hee Jong Koh(高熙宗) 한국육종학회 1988 한국육종학회지 Vol.20 No.4
Eleven primary trisomics of the japonica group, developed and made available by lwata and Omura, were crossed as females with Korean bred Seolakbyo. From the F₁, F₂ and BC₁F₁ of each crosses 11 types, of trisomics were identified on the basis of their distinct morphological characters. Leaf character and plant type were useful criteria for the identification of A, E, F and O type trisomics, while B, C, D, G, H, L and N type trisomics were classified by the panicle and glume characters. The female transmission frequency of the extra chromosome of each trisomics ranged from 8.3 to 35% in F₁, from 16.7 to 50% in F₂ and from 14.3 to 50% in BC₁F₁, respectively in this study.
Quantitative Analysis and Varietal Difference of Cyanidin 3-glucoside in Pigmented Rice
Sun-Zik Park,Jong-Hoon Lee,Sang-Jun Han,Hong-Yeol Kim,Su-Noh Ryu 韓國作物學會 1998 Korean journal of crop science Vol.43 No.3
The cyanidin 3-glucoside (C3G) extracted from pigmented rice seeds in 0.5% TFA (Trifluoro acetic acid) -95% ethanol was separated by High Performance Liquid Chromatography (HPLC). A HPLC system using a Develosil ODS-5 column and 0.1 % TFA-H2O ~0.1 % TFA-CH3CN gradient elution was selected for separation and quantitative determination of C3G. Regression equation obtained for the standard content of C3G pigment was as Y=21.95293* X-14.726771 (r=0.99** ). Using this method, 326 domestic and introduced collections were evaluated for the C3G content. The Korean bred cultivar 'Heugjinjubyeo', showed highest C3G content (552 mg/100g seed) among the tested cultivars. Among the pigmented rice cultivars ten cultivars were selected for containing a high content of C3G. The content of C3G per 100g seeds was in high order as follows: Heugjinjubyeo (552mg)>Cheng Chang (321mg)>Kilimgeugmi (240mg)>PI160979-2 (224mg)>Hong Shei Lo (221mg)>Heugnambyeo (191 mg)>Mitak =PIl60979-1 (186mg)>Suwon425 (163mg)>Sanghaehyanghyeolla (108mg). The C3G pigment was not detected in the common white rice cultivars.
Changes In Special Items And Future Firm Growth
Shin, Young Zik,Lee, Yong Gyu JOURNAL OF APPLIED BUSINESS RESEARCH 2017 JOURNAL OF APPLIED BUSINESS RESEARCH Vol.33 No.5
<P>This study examines the effect of changes in special items on future firm growth. We find that a decrease in special items reverses almost completely through one-year-ahead earnings, whereas an increase in special items is positively associated with the next year’s earnings growth. We also find that the information embedded in changes in special items is incremental to that of fundamental signals documented in the literature, thereby identifying changes in special items as an additional fundamental signal. Furthermore, we find that both an increase and a decrease in special items lead to decreases in future sales and net operating assets, suggesting an inverse U-shaped relation between changes in special items and growth in these measures. Overall, our findings underscore the importance of considering various growth measures in understanding the implication of changes in special items for future firm growth.</P>
Quantitative Analysis and Varietal Difference of Cyanidin 3-glucoside in Pigmented Rice
Park, Sun-Zik,Lee, Jong-Hoon,Han, Sang-Jun,Kim, Hong-Yeol,Ryu, Su-Noh The Korean Society of Crop Science 1998 Korean journal of crop science Vol.43 No.3
The cyanidin 3-glucoside (C3G) extracted from pigmented rice seeds in 0.5% TFA (Trifluoro acetic acid) -95% ethanol was separated by High Performance Liquid Chromatography (HPLC). A HPLC system using a Develosil ODS-5 column and 0.1 % TFA-$H_2O$~0.1 % TFA-$CH_3CN$ gradient elution was selected for separation and quantitative determination of C3G. Regression equation obtained for the standard content of C3G pigment was as Y=21.95293$^*$X-14.726771 (r=0.99$^{**}$). Using this method, 326 domestic and introduced collections were evaluated for the C3G content. The Korean bred cultivar 'Heugjinjubyeo', showed highest C3G content (552 mg/100g seed) among the tested cultivars. Among the pigmented rice cultivars ten cultivars were selected for containing a high content of C3G. The content of C3G per 100g seeds was in high order as follows: Heugjinjubyeo (552mg)>Cheng Chang (321mg)>Kilimgeugmi (240mg)>PI160979-2 (224mg)>Hong Shei Lo (221mg)>Heugnambyeo (191 mg)>Mitak =PIl60979-1 (186mg)>Suwon425 (163mg)>Sanghaehyanghyeolla (108mg). The C3G pigment was not detected in the common white rice cultivars.
C3G색소 고함유 벼품종의 자유라디칼 소거작용 및 항염효과
박순직,류수노,Park, Sun-Zik,Ryu, Su-Noh 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1
국내육성 흑자색미(흑진주벼, 흑남벼, C3GHi벼)와 일반미(일품벼, 추청벼, 동진벼) 품종의 메탄을 추출물을 대상으로 항산화 항혈전 항염증활성을 검토하였으며, 그 결과는 다음과 같다. 1. 0.4 mg/mL농도에서 C3GHi벼와 흑진주벼 추출물이 73.25%, 50.38%의 우수한 자유라디칼 소거능을 나타내었다. 2. 메탄올추출물의 혈액응고 저해활성을 측정한 결과, C3GHi 벼는 258.76%, 흑진주벼는 243.52%의 강한 트롬빈 저해활성을 보였다. 그러나 대조약물로 사용한 아스피린보다(398%) 약하게 나타났다. 3. 공시한 흑자색미와 일반미 품종들은 COX-1과 COX-2에 대한 활성이 나타나지 않았으나, C3GHi벼는 PD2에 대한 항염활성을 보였다. 4. H. pylori균에 의해서 나타나는 AGS cell line에 대한 독성을 조사한 결과 흑자색미 추출물은 $250{\mu}g$과 $500{\mu}g$ 처리군에서 모두 H. pylori균의 AGS cell line에 대한 부착을 억제하는 활성이 있는 것으로 판명되었으며 일반미 추출물은 H. pylori 균에 대한 저해작용은 없는 것으로 나타났다. 5. Zone assay에 의한 항균실험결과 흑자색미 추출물은 $200{\mu}g/mL$의 농도에서 ATCC 43504 및 COO1 균주에 대해서 clear zone이 검출되어 항균활성이 있는 것으로 판명되었으나 SEO 균주에 대해서는 항균활성이 검출되지 않았다. Free Radical Scavening and inflammatory of the methanol extracts, which were prepared from 6 difffrent bran of rice, were evaluated to investigate bioactive substances. Among them, the extract of C3GHibyeo and Heugjinjubyeo showed strong DPPH scavening activities (73.25% and 50.38% at 0.4 mg/ml, respectively). The extract of C3GHibyeo and Heugjinjubyeo showed strong thrombin inhibition activities (258.76% and 243.52% inhibition at 5 mg/ml, respectively). The result of antibacterial activity by Zone assay showed that C3GHi rice extract $(250{\mu}g\;and\;500{\mu}g)$ inhibited attachment of Helicobactor pylori on the ATCC48504 and COO1 cell line. But no effect on the SEO cell line. Cytotoxicity of blackish purple rice extract on the H. pylori doesn't showed. These result support a functional superiority of rice-base livelihood, and suggest that the development of healthy food using functional ingredients of rice is possible.
Determination of C3G Content in Blackish Purple Rice using HPLC and UV-Vis Spectrophotometer
Su Noh Ryu,Sun Zik Park,Sam Sik Kang,Sang Jun Han 韓國作物學會 2003 Korean journal of crop science Vol.48 No.5
Cyanidin 3-glucoside (C3G) content contained in the grains of blackish purple rice varieties, Heugjinjubyeo, Kilimheugmi, Heugnambyeo, Sanghaehy-anghyeolla, and the progenies derived from their crosses was evaluated by HPLC and UV-Vis spectroscopy. C3G content was higher in the range of 10-30% by using UV-Vis method compared to HPLC method. A significant linear relationship was, however, observed between two analytical methods. The correlation coefficient was 0.98. Thus, this results suggested that it would be able to use UV-Vis spectroscopy to determine C3G content which does not demanded precise value like selection.